ResourceLife Sciences
High-sensitivity ATP Quantitation using SpectraMax Injector Cartridge with SmartInject Technology
High-sensitivity ATP Quantitation using SpectraMax Injector Cartridge with SmartInject Technology
22 Oct 2015Adenosine 5´-triphosphate (ATP) is present in all living cells. Because the level is strictly controlled, the assay of ATP can be used as an indicator of viable cell number. ATP measurements are used to monitor raw materials and manufacturing plant hygiene for bacterial contamination as well as for waste water analysis. In the biotechnology and pharmaceutical industries, ATP measurements are used to evaluate cell proliferation and cytotoxicity. This application note demonstrates highly sensitive detection of ATP down to 20 amol/well and a dynamic range of 5 decades using the SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge and SmartInject™ Technology.
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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.RegulatoryPharmaceutical regulations impact on all areas of drug development, manufacture and control and supply. Services are provided from the early stages of drug development and clinical trials through to dossier submission, approval and marketing. Regulatory also includes submission of analytical studies from the pharmaceutical product and safety. CytotoxicityCytotoxicity assays measure the toxic effects of substances on cells, often used in drug testing and environmental studies. These tests are crucial in determining the safety of chemicals and pharmaceutical compounds. Explore cytotoxicity testing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Waste WaterContamination PreventionContamination prevention aims to control and minimize the risk of contamination during experiments. Biological safety cabinets and sterilization equipment are examples of technologies used to prevent contamination.ATP MonitoringBioluminescenceFood ContaminationFood contamination refers to the presence of harmful substances or pathogens in food, posing risks to human health. Contaminants can be biological, chemical, or physical, and their detection is crucial for ensuring food safety. Advanced testing methods like PCR, chromatography, and spectroscopy are used to identify contaminants in food products. Browse our peer-reviewed product directory to find the best solutions for food contamination detection, compare products, check reviews, and get pricing directly from manufacturers.Cell ProliferationCell proliferation refers to the process by which cells divide and multiply. This is a key concept in cancer research, tissue regeneration, and immunology studies. Explore cell proliferation tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Bacterial DetectionBacterial detection uses methods such as cell culture, microscopy, and biochemical assays to identify harmful or beneficial bacteria based on their morphological and metabolic charactersistics. These techniques support disease diagnostics, food safety, and microbial research. Explore bacterial detection systems, kits, and consumables in our directory to compare products and read reviews.