High-Cell-Density Cultivation of Escherichia coli in a BIOSTAT® D-DCU 10-3 Stainless Steel Bioreactor
29 Oct 2014Generally, stainless steel bioreactor systems are used for microbial fermentations because their design principles are well known. Consequently, there is a lot of experience and knowledge for these bioreactors resulting in their use over several decades. Furthermore, such bioreactors are available in a scalable range from laboratory to production scale, which enables easy process transfer and scale-up. BIOSTAT® D-DCU is a bioreactor for both microbial and cell culture applications, offering the capability of sterilization in place (SIP). This application note demonstrates the high-cell-density cultivation of Escherichia coli in a BIOSTAT® D-DCU 10-3 Bioreactor.
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BIOSTAT® D-DCU
Sartorius GroupThe BIOSTAT® D-DCU is a compact, bioprocess system available for microbial and cell culture applications with working volumes from 10 to 200L. The BIOSTAT D-DCU incorporates many desirable and advanced features to fulfil virtually any demand for modern bioprocess application, such as: Automatic Sterilization in Place (SIP) and Cleaning in Place (CIP), Dual pH and DO measurement capability, Lid lifting device, WIT-Ready filter housings, Dual exhaust filter housing line, Tube and shell exhaust cooler, exhaust heater, automatic or manual addition arrays as well as other accessories. Furthermore the BIOSTAT D-DCU is designed to interface Single use storage bags for media addition and harvest as well as single use sampling systems. Due to the modular approach, resulting in various configurations; from baseline to fully-featured, the system offers an excellent solution for every budget and every need.Features: Single or Twin configuration 10L, 20L, 30L, 50L, 100L, and 200L working volume For microbial or cell culture applications Automated Sterilization in Place (SIP) Automated Cleaning In Place (CIP) Space saving design Full qualification support