From shaker to fermenter, a methanol-free Pichia pastoris protein production workflow

5 Sept 2025

Pichia pastoris is widely employed for producing recombinant proteins. Traditional systems depend on methanol-inducible promoters like PAOX1, which pose safety concerns due to methanol’s flammable nature.

Find out more about a new strain of P. pastoris that carries a glycerol-inducible promoter, pUPP, that enables safe, methanol-free expression of proteins under glycerol feeding for high‑density fed‑batch fermentation.

Eppendorf BioFlo® 320

Eppendorf

 The BioFlo 320 seamlessly blends beauty and utility in one all-inclusive package. A new industrial design, flexibility between interchangeable autoclavable and single-use vessels, intelligent sensors, Ethernet connectivity, and a new software package are only a few of the features that set it apart from the competition

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Eppendorf BioFlo® 120

Eppendorf

The Eppendorf BioFlo 120 offers simplicity and ease of use, without sacrificing capability. In academic, governmental or industrial research settings; for work with bacteria, yeast, fungi, mammalian, insect or plant cells: The BioFlo 120 is an attractive solution to meet bioprocessing needs. The BioFlo 120 is a bench-scale bioreactor/fermentor system that perfectly suits research and development. Its flexible design meets the wide-ranging needs of scientists in both microbial and cell culture applications. 24 interchangeable autoclavable vessels (water-jacketed or with heat blanket) are available, along with BioBLU® single-use bioreactors ranging from 250 mL to 40 L working volume. Digital Mettler Toledo® ISM sensor technology is fully integrated allowing the user to switch between pH, redox, dissolved oxygen (polarographic and/or optical), and carbon dioxide monitoring without the need for additional equipment. A high-precision thermal mass flow controller with expanded range provides the capability for low flow mammalian cell culture through high-demand microbial processes on a single control station.   Features: Scale-up from 250 mL to 40 L on a wide variety of autoclavable and single-use vessels New Auto culture modes for microbial and cell culture process control at the touch of a button. Universal connections for analog sensors or digital Mettler Toledo ISM sensors for increased flexibility Applications: Cultivation of mammalian, stem, insect, and plant cells Bacterial, yeast, and fungal fermentation Batch, fed-batch, continuous or perfusion

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From shaker to fermenter, a methanol-free Pichia pastoris protein production workflow