From shaker to fermenter, a methanol-free Pichia pastoris protein production workflow

Pichia pastoris is widely employed for producing recombinant proteins. Traditional systems depend on methanol-inducible promoters like PAOX1, which pose safety concerns due to methanol’s flammable nature.

Find out more about a new strain of P. pastoris that carries a glycerol-inducible promoter, pUPP, that enables safe, methanol-free expression of proteins under glycerol feeding for high‑density fed‑batch fermentation.