From data-independent acquisition (DIA) to targeted MS/MS: Automatic reinjection for additional confirmation in suspect screening

Multi-residue screening in food safety is challenged with an ever-growing list of regulated residues.To comply with SANTE guidelines for the simultaneous identification and quantitation of compounds, overlapping molecular ions and a specified number of fragments are required over the chromatographic peak. DIA workflows fulfill these requirements but can yield questionable results. In this poster, Agilent Technologies presents the concept of an intelligent reflex, where after the initial sample run in DIA mode (All Ions) the data is processed, and automatically appended to the running worklist to confirm questionable or identified residues via targeted MS/MS. Agilent Technologies demonstrates in this study that the Revident LC/Q-TOF is highly suitable for routine screening in food safety applications.