Fabricating Large Scale Biomolecular Nanoarrays

Dip Pen Nanolithography® (DPN®) is an established method of nanofabrication in which materials are deposited onto a surface using a sharp tip. In this application note from NanoInk DPN was used to fabricate arrays of nanometer-scale features that served as attachment points for thiolated biomolecules. These binding sites were fabricated using a block co-polymer to transport small amounts of gold ions to defined locations on to a substrate. The printed droplets were heat treated to form gold nanoclusters at each deposition location, and were subsequently capable of tethering both protein and DNA molecules.