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Extraction of Testosterone from Serum using Oasis Max μElution Plates for Clinical Research

13 Jan 2015

This application note describes a reproducible LC-MS/MS method for the analysis of testosterone for clinical research. Sample preparation was performed using an Oasis MAX 96-well μElution Plate for the extraction of testosterone from serum. Results demonstrate analytically sensitive analysis of testosterone in serum (LLOQ 0.17 nmol/L).

Oasis® µElution Plate

Waters

The Oasis® uElution plate format combined with the Oasis chemistries allow elution volumes as low as 25uL, eliminating the time consuming evaporation step, producing very clean extracts with a 5 to 10 fold increase in concentration.The Oasis® family of sample extraction products is designed to simplify and improve your sample preparation by combining the right sorbent chemistry, device format and methodology. Achieve robust, selective, and sensitive solid-phase extraction [SPE] methods without worrying about low recoveries caused by breakthrough, sorbent drying, pH limitations, and undesirable silanol activity.Oasis® SPE sorbents—covered by eight U.S. patents—are unique in their purity, reproducibility, stability, and retention characteristics. Today, they are the most widely used polymeric SPE products in bioanalytical laboratories.

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Extraction of Testosterone from Serum using Oasis Max μElution Plates for Clinical Research