ResourceLife Sciences
Evaluation of a New Intracellular Calcium Fluorescent Reagent Using the FDSS6000
22 Dec 2014In this application note, the fluorescence spectral properties of DiscoveRx Calcium assay reagent are evaluated, and are compared with the parameters of the FDSS6000 calcium assay filter set. Data obtained in several experimental conditions are presented, and allow the assessment of DiscoveRx Calcium Assay reagent and Hamamatsu FDSS6000 reader as an appropriate environment for running GPCR calcium release functional HTS.
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.