Enhancing MRM Experiments in GC/MS/MS Using APGC

8 Oct 2013

For many food testing labs, both GC and LC are important techniques that enable a broad range of compounds to be analysed. The Xevo TQ-S in combination with both the ACQUITY UPLC and APGC makes it an attractive MS for routine food testing laboratories due to its chromatographic versatility, the ability to choose more selective MRMs, and the system sensitivity. This application note evaluates the potential of APGC–Xevo TQ-S for the quantitative analysis of pesticides in foodstuffs.

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Waters Xevo™ TQ MS provides the ultimate in quantitative capabilities for your most demanding applications and your most complex sample matrices. It is a highly advanced tandem quadrupole mass spectrometer designed to enable uncomplicated access to superior HPLC and UPLC®/MS/MS performance, delivering the highest levels of sensitivity, selectivity, robustness, speed, and accuracy. Waters Xevo TQ MS is equipped with unique collision cell technology (ScanWave™) to provide new enhanced spectral LC/MS/MS data acquisition capabilities. Unlike conventional tandem quadrupole mass spectrometers, the Xevo TQ MS is designed not only to provide the very best quantitative data, but also to provide you with superior spectral MS/MS information. Xevo TQ MS offers you much more versatility in a single instrument platform that is adaptable to a variety of different UPLC/MS/MS applications both quantitative and qualitative: bioanalysis, ADME screening, food safety, environmental monitoring, forensic, and more. Features: Automated system set up and system checks for simple access Automated instrument optimization and quantitative method development tools for ease-of-use The latest innovations in high efficiency, robust, easy-to-use ZSpray™ atmospheric pressure ion source technology Versatile range of ionization options, including multi-mode ionization capability in a single run Unique, innovative T-Wave™ and ScanWave enabled collision cell technology Footprint that sits easily on your laboratory benchtop Dedicated application managers for your specialized analytical requirements
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ACQUITY UPLC System
Waters
Waters ACQUITY UPLC - Dramatic Improvements in Speed, Sensitivity and Resolution Whether you work in methods development or quality assurance, in drug discovery and development or testing for safety in food supplies, you seek a productivity edge. With the ACQUITY UPLC® System, now you can get more information in a single, short run than you've ever seen with HPLC. Waters ACQUITY UPLC System UltraPerformance LC® (UPLC®) technology starts with unique 1.7 μm small-particle chemistries. Chromatographers no longer need to choose between speed and resolution–with UPLC you get both. Instrument Designed for Chemistry; Chemistry Designed for the Instrument The ACQUITY UPLC System has been holistically designed to match the performance needs of our innovative column chemistries with robust hardware, easy-to-use software and specialized support services. Small, pressure-tolerant particles High-pressure fluidic modules Minimized system volume Negligible carryover Reduced cycle times Fast response detectors Integrated system software and diagnostics With UPLC you have the ability to work more efficiently with higher speed, sensitivity and resolution at a much wider range of linear velocities, flow rates and backpressures to obtain superior results. The Waters ACQUITY UPLC System's high-pressure fluidics optimizes flow rates to make the most of small particle technology. The ACQUITY UPLC System's sample-handling design is designed to ensure exceptionally low carryover and reduced cycle time. And when interfaced with the Sample Organizer, it increases unattended sample capacity by ten times. High-speed detectors, both optical and mass, contribute to increased sensitivity and help manage the heightened speed and resolution requirements of UPLC. ACQUITY UPLC Systems are easily controlled, diagnosed, and monitored via a graphical system console interface. The console offers: Quick and easy access to critical instrument parameters Simple system start-up, elegant system status monitoring and predictive performance indicators to ensure maximum productivity Data management capabilities that are supported by both MassLynx™ and Empower™ software The ACQUITY UPLC System is also supported by Intelligent Device Management technology with our Connections® INSIGHT™ service, providing instrument diagnostics.
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MassLynx MS Software
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Acquire, Analyze, Manage, and Share Mass Spectrometry Information MassLynx™ Software increases the speed at which you can convert your sample data into valuable knowledge. It provides you with the fundamental platform to acquire, analyze, manage, and share your mass spectrometry information. MassLynx intelligently controls any Waters mass spectrometry system, from sample and solvent management components to mass spectrometer and auxiliary detectors. MassLynx Software may acquire nominal mass, exact mass, MS/MS and exact mass MS/MS data. MassLynx’s Sample List is the core of the system. It maintains and consolidates the data on all of your samples. You also initiate any activities related to your sample from the Sample List. This "sample centric" approach simplifies the interaction with your LC/MS or GC/MS system, acquired data and processed results. Process Application-specific Data MassLynx Software features general purpose and specialized Application Managers that provide information for your specific MS analyses and data. Two come standard with MassLynx: QuanLynx™ for automated quantification included as standard with MassLynx OpenLynx™ for qualitative screening and identification Optional Application Managers can perform: Targeted quantitative analysis Mass-directed purification Metabolite identification Deconvolution of complex chromatograms Metabonomics & metabolomics Protein identification and protein characterization
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TargetLynx
Waters
The TargetLynx™ Application Manager automates sample data acquisition, processing and reporting for quantitative results.It incorporates a range of confirmatory checks that identify samples that fall outside user-specified or regulatory thresholds. Identify Non-compliant Samples TargetLynx rapidly identifies and flags samples where: Analytes are above a specified maximum reporting level (MRL) Analyte confirmatory ion ratios are outside specified limits One or more analyte signal-to-noise ratios are below a defined value An analyte retention time or relative retention time is outside limits An analyte concentration is below set LOD and LOQ thresholds The standard deviation of response for QC standards exceeds a defined value The blank response is too high The coefficient of determination (r2) of the calibration curve exceeds a defined value LC/MS & LC/MS/MS and GC/MS & GC/MS/MS Use TargetLynx to quantify and confirm both LC/MS & LC/MS/MS and GC/MS & GC/MS/MS data. This data may be selected ion monitoring (SIM), multiple reaction monitoring (MRM), or full scan/full spectrum. In the case of full scan/spectrum data, extracted ion chromatograms are used for quantification. Ensure Regulatory Compliance The demand by regulatory authorities for assurance of confirmation in quantitative analysis continues to increase. To ensure cost-effective compliance, TargetLynx offers software that gives you confidence in your results and eliminates time-consuming, costly manual checks.
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ACQUITY UPLC System

Waters

Waters ACQUITY UPLC - Dramatic Improvements in Speed, Sensitivity and Resolution Whether you work in methods development or quality assurance, in drug discovery and development or testing for safety in food supplies, you seek a productivity edge. With the ACQUITY UPLC® System, now you can get more information in a single, short run than you've ever seen with HPLC. Waters ACQUITY UPLC System UltraPerformance LC® (UPLC®) technology starts with unique 1.7 μm small-particle chemistries. Chromatographers no longer need to choose between speed and resolution–with UPLC you get both. Instrument Designed for Chemistry; Chemistry Designed for the Instrument The ACQUITY UPLC System has been holistically designed to match the performance needs of our innovative column chemistries with robust hardware, easy-to-use software and specialized support services. Small, pressure-tolerant particles High-pressure fluidic modules Minimized system volume Negligible carryover Reduced cycle times Fast response detectors Integrated system software and diagnostics With UPLC you have the ability to work more efficiently with higher speed, sensitivity and resolution at a much wider range of linear velocities, flow rates and backpressures to obtain superior results. The Waters ACQUITY UPLC System's high-pressure fluidics optimizes flow rates to make the most of small particle technology. The ACQUITY UPLC System's sample-handling design is designed to ensure exceptionally low carryover and reduced cycle time. And when interfaced with the Sample Organizer, it increases unattended sample capacity by ten times. High-speed detectors, both optical and mass, contribute to increased sensitivity and help manage the heightened speed and resolution requirements of UPLC. ACQUITY UPLC Systems are easily controlled, diagnosed, and monitored via a graphical system console interface. The console offers: Quick and easy access to critical instrument parameters Simple system start-up, elegant system status monitoring and predictive performance indicators to ensure maximum productivity Data management capabilities that are supported by both MassLynx™ and Empower™ software The ACQUITY UPLC System is also supported by Intelligent Device Management technology with our Connections® INSIGHT™ service, providing instrument diagnostics.

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Enhancing MRM Experiments in GC/MS/MS Using APGC

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Xevo TQ MS

ACQUITY UPLC System

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TargetLynx

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