Efficient Plasmid DNA Isolation from Bacterial Culture with Thermo Scientific KingFisher Flex and KingFisher Pure Plasmid Kit

14 Jan 2015

Efficient plasmid DNA purification from bacterial cultures is key to many molecular-based experiments. This application note describes an automated process for plasmid DNA purification from bacterial cultures. The method integrates the use of the Thermo Scientific™ KingFisher™ Pure Plasmid Kit and the KingFisher Flex platform for efficient purification of plasmids of various sizes. The system was also used to purify plasmids containing recombinant antibody sequences for sequence identification.

Thermo Scientific KingFisher Flex

Thermo Fisher Scientific

The Thermo Scientific KingFisher Flex magnetic particle processor offers great flexibility for high-throughput purification of DNA/RNA, proteins and cells from virtually any source.Using patented magnetic particle separation technology, the KingFisher Flex provides a fast and easy way to get high yields of purified samples with excellent reproducibility and quality.Key Features: High-throughput system processes up to 96 samples in 30 minutes Flexible, open system lets you customize your own purification process Patented magnetic particle processing technology lets you set your protocols and walk away Application Areas: Genetic testing Microbial identification Plant genetics Evolutionary studies / Phylogenetics Protein identification Immunology

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Thermo Scientific KingFisher Pure Kits

Thermo Fisher Scientific

The Thermo Scientific KingFisher Pure Kits are specially designed for use with the KingFisher nucleic acid purification system. These optimized reagent kits help you achieve rapid, high-quality results for a variety of applications – with minimal effort. KingFisher Pure Kits are now available for Cell and Tissue DNA, DNA Blood, DNA Plant, Plasmid, RNA Blood, RNA Plant, RNA Tissue, and Viral NA.Key Features: Developed specifically for use with KingFisher magnetic particle processors Optimized for DNA/RNA purification from a variety of sources Easy-to-use system for pure performance, every time Application Areas: Genetic testing Microbial identification Plant genetics Evolutionary studies / Phylogenetics

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Efficient Plasmid DNA Isolation from Bacterial Culture with Thermo Scientific KingFisher Flex and KingFisher Pure Plasmid Kit