Efficient analysis of sgRNA and its impurities by IP-RP using YMC Accura Triart Bio C18

This application note demonstrates a high‑resolution method for the analysis of single guide RNA (sgRNA) and closely related impurities using ion‑pair reversed‑phase (IP‑RP) HPLC. The approach addresses key analytical challenges associated with sgRNA, including the separation of structurally similar truncation variants and the impact of secondary structure on chromatographic performance.

Discover how by using a bioinert YMC Accura Triart Bio C18 column, the method enables efficient separation of full‑length sgRNA from n–1, n–2, and n–5 impurities, even at low concentrations. The study highlights improved resolution, minimal carry‑over, and reproducible performance, supporting robust purity assessment for gene editing applications.