Effective Sample Preparation and Rapid LC/MS/MS Analysis of a Large Pain Panel Screen Using Phenomenex Columns

Using a single Solid Phase Extraction (SPE) method on a specialized sorbent, 41 pain management drugs from several drug classifications were isolated from urine samples and analyzed by LC/MS/MS in under 5 minutes using a Kinetex Core-Shell Technology HPLC/UHPLC column. The Strata-X-Drug B SPE sorbent was not only able to successfully extract the entire panel of pain management drugs under investigation but it also helped to reduce the amount of time and solvent required for analysis since the sorbent does not require a condition or equilibration step.