Dynamic evaluation of transgene expression and transfection performance using real-time live cell analysis system

This application note demonstrates how the Spica M1 real-time live-cell analysis system enables dynamic, multi-parameter evaluation of transgene expression and transfection performance. Using GFP reporter assays in 293T cells, the study highlights continuous monitoring of expression kinetics, expressivity, penetrance, and cell viability, revealing critical insights that traditional endpoint assays miss. See how the Spica M1 supports data-driven optimization of transfection conditions while preserving cell health, accelerating gene expression research, and cell line development.