Development of a Medium Throughput Electrophysiology Assay to Investigate State-Dependency of Sodium Channel Blockers Using QPatch
3 Apr 2011This application note evaluates the suitability of the CHO ChanClone™ Nav1.5 cell line, over-expressing recombinant human Nav1.5 (a voltage-gated sodiumchannel), for use on the automated electrophysiology platform, QPatch. The researchers develop an assay suitable for the investigation of pharmacological modulators of Nav1.5 channels. The automated electrophysiology platform is capable of producing data of equivalent quality to that obtained using manual patch, but at significantly higher throughput (up to 16 cells can be patched in parallel).
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QPatch HT and QPatch HTX
Sophion BioscienceThe QPatch HT and QPatch HTX enable high-throughput patch clamping with 48 parallel recording sites. Automated patch clamping with the same high quality afforded by conventional patch clamping can now be applied in all phases of ion channel drug discovery, including primary screening of focused compound libraries. • Up to 7000 data points per day • Screening and compound profiling • Multi-hole and single-hole mode (QPatch HTX) • Ligand- and voltage-gated recordings • Eight pipette liquid handling with intelligent scheduler • True gigaseals • Flow channels for solution exchange • Up to 50 liquid additions • User-friendly experiment execution • Unattended operation for several hours • On-board cell preparation • Elevator units for QPlate stacking • Elevator units for compound plate stacking • Cell clone screening feature • Multiple intracellular solutions testing feature • User friendly assay setup and quick data analysis