ResourceSeparations

Developing fast purification methods for natural products

4 Nov 2021

In this application note, a fast and simple purification method for withaferin A in ashwagandha extract is detailed using an Agilent InfinityLab Poroshell 120 SB-C18 preparative LC column. Explore how the speed and loadability of the InfinityLab Poroshell column make it well suited to the needs of high-throughput discovery laboratories.

Poroshell 120

Agilent Technologies

Poroshell 120 columns provide rugged, high resolution separations within the pressure range of any mainstream LC system, making the benefits of sub-2 µm performance available with all existing LCs.Poroshell columns can achieve high resolution and high speed separations on current instruments, and higher resolution and speeds on new high-pressure LC and LC/MS systems. Stable to 600 bar, Poroshell 120 are great for fast UHPLC analyses, and can accelerate your older, traditional 5 µm method for 4-5x reduction in analysis time. With a family of ten chemistries, including the recently introduced PFP, and alignment with the ZORBAX family chemistries, method development and transfer is flexible and easy.Features: High efficiency and high resolution, with up to 50% less backpressure than sub-2 μm columns 2 μm frit, for rugged performance with dirty samples Compatible with 400 bar and 600 bar LCs, as well as UHPLC instruments An expanding family of bonded phases to align with the ZORBAX Family, for reliable scalability Excellent selectivity and peak shapes Designed for exceptional reproducibility Explore Poroshell 120 solutions focused applications, from food to pharma

(18)

Links

Tags

Developing fast purification methods for natural products