Determination of Inhibitor Residence Times for a Phosphodiesterase using the Transcreener® AMP2/GMP2 FP Assay
18 Aug 2015The Transcreener® assays rely on antibodies that are able to differentiate between nucleotides on the basis of subtle structural differences. This makes detection of the product nucleotide possible even in the presence of excess substrate nucleotide. This case study focuses on the biochemical characterization of a broad range of targets, using Jump Dilution protocol to make residence time determinations for Transcreener® assays.
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Transcreener® AMP²/GMP² Phosphodiesterase Assays
BellBrook LabsThe Transcreener® AMP²/GMP² Phosphodiesterase Assay Kit directly detects AMP or GMP produced by PDEs, ligases, synthetases, ENPP1, and related enzymes using a mix‑and‑read FP or TR‑FRET format—no coupling steps needed. Compatible with 1–1000 µM native substrates, it delivers excellent HTS performance (Z’ ≥ 0.7, ≥100 mP shift) in a one-step, far‑red fluorescence assay.