Detailed glycosylation analysis of therapeutic enzymes using µPAC capLC-MS and all-ion fragmentation
25 Feb 2021In this application note, the use of micro Pillar Array Columns (μPAC™) for characterizing glycosylation of therapeutic enzymes is presented. Recombinant human acid α-glucosidase (hGAA) was digested and resulting peptides were separated on a 50 cm μPAC™ capLC C18 column operated at low μL/min flow rate. Glycopeptide peaks were then selectively detected and identified by Orbitrap mass spectrometry (MS) operated in full MS/all-ion fragmentation (AIF) mode.
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µPAC™ capLC column
PharmaFluidicsThe µPAC™ capLC columns are designed for increased robustness and throughput assuring sensitivity. A flow rate versatility between 1 and 15 µL/min at moderated pressures allows short gradient separations, while the µPAC™ technology ensures an exceptional high reproducibility over time and across laboratories. µPAC™ column, 50cm length, with pillar array backbone at interpillar distance of 2.5 µm, C18.