Delivery of genome editing tools to knockout target genes

18 Aug 2021

Genome editing is a technology that enables specific editing of any sequence in an organism's genome (knockout, knock-in, single nucleotide editing, etc.). This application note introduces the features of the SU10 and provide examples demonstrating the delivery of genome editing tools (Cas9 RNP) using the technology.

Single Cellome™ Unit SU10

Yokogawa Corp. of America

SU10 is a nanopipette-based tool for efficient and controlled delivery of material (such as CRISPR Cas9 complex, RNA, proteins) into the nucleus or cytoplasm of single cells in a highly automated manner. The CRISPR Cas9 complex can be delivered directly into the nucleus of a specific target cell in a minimally invasive manner through the utilized glass pipette in nanometer range (approx.100 nm).

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Delivery of genome editing tools to knockout target genes