ResourceLife Sciences
Comprehensive Comparison of Protein Microarray Supports using Contact and Non-Contact Systems
23 Aug 2017The quality of biochips depends on both the support membrane and the deposition technology. This application note compares Nitrocellulose Membranes (NC) Sartorius white (SCHOTT Nexterion Slides NC-W) and Sartorius black (SCHOTT Nexterion Slides NC-D*), to other comerically available NC slides. The probe deposition was performed using a split-pin spotter (Genetix Qarray mini), pin and ring spotter (Affymetrix 417 Arrayer) and a non-contact spotter (sciFlex Arrayer S3).
Links
Tags
ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NanoarrayPrecision Liquid HandlingProtein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.
