Rapid analysis of 17 bile acids in human plasma by LC-MS/MS

The quantitative analysis of bile acids in plasma is critical for diagnosing many structural liver diseases. Accurate reporting can be difficult because of analyte characteristics, matrix effects, and other factors. In this work, we describe a robust, selective LC-MS/MS method for the analysis of 17 bile acids in under 10 minutes, including three isomer groups, with special attention given to a matrix interference observed in routine human plasma analysis.

In this work, Cambridge Isotope Laboratories describes a robust, selective LC-MS/MS method for the analysis of 17 bile acids in under 10 minutes, including three isomer groups, with special attention given to a matrix interference observed in routine human plasma analysis.