ResourceLife Sciences
Channel- & Compensation-free Dead Cell Exclusion: N+1 Panel Design
19 Jan 2018Dead cell exclusion is a common requirement for flow cytometry using viability dyes like DAPI, PI and 7-AAD. Anew far-red fluorescing alternative DRAQ7™ has been robustly tested showing improved separation between negative and positive events. Conventional flow cytometry is limited by spectral "space" and the preference for bright chromophores to label antibodies to aid sensitivity that increases channel spillover. At its simplest, far-red DRAQ7 can benefit panel design where, for example, the complete absence of spillover into the FITC and R-PE detectors makes it an obvious replacement for 7-AAD in CD34+ cell enumeration.
Related products
Request Quote for All Products
Links
Tags
Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Cell BiologyCell biology studies the structure, function, and behavior of cells. Understanding cellular processes is essential for research in areas such as cancer biology, stem cell research, and neurobiology. Techniques like flow cytometry, microscopy, and cell culture enable researchers to explore cellular mechanisms in detail. Browse our peer-reviewed product directory to find the best cell biology tools and equipment, compare products, check customer reviews, and get pricing directly from manufacturers.