Cell Sorting Based on RNA detection in Living Cells Using SmartFlare™ RNA Detection Reagents
14 Apr 2013Identifying cell types and sorting cells based on RNA expression levels without any transfection reagents or intrusive sample preparation can drastically improve live cell sorting efficiency, physiological relevance, and post-sorting survival rate. In this application note it is demonstrated that SmartFlare™ RNA detection probes, which are capable of detecting levels of RNA inside living cells, can be used to sort and further propagate live cell populations purely based on gene expression levels or in combination with surface markers detected with antibodies.
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SmartFlare RNA Detection Probes
MerckSmartFlare Probes work by recognizing specific native RNA sequences within living cells, therefore there are many possible sequences that you could design to recognize your target of interest. Instead of lysed cells, switch to live cells. And while you’re at it, eliminate sample preparation and transfection steps all together. Live cell RNA detection is now possible, in a single incubation step using inert nanoparticle technology to specifically detect native RNA. And when you’re done, the probes exit the cells allowing you to perform downstream analyses with the same, unperturbed cells. Make the smart change!SmartFlare RNA Detection Probes are fueled by the NanoFlare technology developed by renowned chemist Dr. Chad Mirkin.What's Smart? Detect RNA expression in live cells for real time, physiologically relevant data Eliminate laborious, costly sample preparation Easy analysis on the fluorescent detection platform of your choice Nanoparticle based technology that allows cells to be used for downstream assays