ResourceLife Sciences
Cell-Based Fluorescence Assay for Selectivity Evaluation between Two Na<sup>+</sup> Channel Subtypes
2 Feb 2015Sodium voltage gated ion channels are expressed in central neurons. Certain subtypes have been implicated in different forms of epilepsy and as such, have been targets in the development of anti-epileptic drugs. Very few methods are available to study voltage gated ion channels in high throughput which has impaired drug discovery for epilepsy. This application note describes a fluorescence method, scalable in high throughput, based on the use of SBFI dye in the Hamamatsu FDSS6000 screening system.
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.ElectrophysiologyElectrophysiology is the study of biological voltage changes, from a single ion channel through to a whole organ scale. Voltages can be measured using patch clamping, automated workstations, electrophysiology rigs or cell based assays. Specific cell lines, manipulators and microinjectors are also available for electrophysiology research.Ion ChannelHigh ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.