ResourceLife Sciences
BRET1-Assay Using the FDSS/µCELL Imaging Plate Reader
11 Dec 2014The GPCRs represent the largest family of cell surface receptors and are the main target for drugs available on the pharmaceutical market. The biophysical technique named Bioluminescunce Resonance Energy Transfer (BRET) has been widely used to monitor and quantify agonist-promoted β-arrestin recruitment. This BRET application demonstrates monitoring the activity of the dopaminergic D2 receptor using the FDSS/µCELL imaging plate reader.
Links
Tags
Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.ReceptorsgPCR