Balanced sequencing pools through controlled amplification using AutoNorm™

This application note demonstrates how iconPCR^™ technology with AutoNorm^™ on the icon96 real-time thermocycler enables balanced sequencing pools by dynamically controlling PCR amplification in each well. Unlike conventional fixed-cycle PCR workflows that require post-PCR quantification and normalization, AutoNorm monitors fluorescence in real time and stops amplification once a target threshold is reached. This automated normalization produces consistent library yields across variable inputs, simplifies NGS library preparation, reduces hands-on time by up to 60%, minimizes failed libraries, and maintains balanced read distribution for whole genome sequencing.