Automated qPCR master mix preparation with the D-ONE single channel pipetting module and the ASSIST PLUS pipetting robot

23 Feb 2023

Automating qPCR master mix preparation is often considered tedious due to the need to constantly change program set-up based on varying master mix volumes and sample numbers. This application note demonstrates how the D-ONE single channel pipetting module for the ASSIST PLUS pipetting robot is designed to simplify the programming of automated qPCR master mix preparation protocols down to a single step. Here, the D-ONE and ASSIST PLUS is used to verify a SYBR® Green qPCR protocol prepared directly in a divided reservoir.

D-ONE

INTEGRA Biosciences

The D-ONE single channel pipetting module offers hands-free transfers from individual tubes or wells on the ASSIST PLUS pipetting robot. This makes it the perfect add-on for labs that want to go beyond the possibilities of multichannel pipetting to access unlimited applications. It is designed to offer easy automation of normalization, hit picking and master mix preparation, freeing up your time while eliminating transcription errors.

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Automated qPCR master mix preparation with the D-ONE single channel pipetting module and the ASSIST PLUS pipetting robot