Analysis of Sugars in Orange Juice and Grape Juice by Prominence-i and Differential Refractive Index Detector
9 Oct 2014This application note introduces an example of sugar analysis in juices using the Prominence-i integrated high-performance liquid chromatograph with the RID-10A differential refractive index detector. Since the column oven can accommodate a 30-cm column for use in sugar analysis (ligand exchange column), and the temperature can be controlled up to 85 °C, it therefore supports applications that require a long column and high column temperature.
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Prominence HPLC System
The Shimadzu Prominence high-performance liquid chromatograph achieves an exceptional level of performance, reliability and great expandability to support diverse applications from ultra-fast liquid chromatography to preparative LC, gel permeation chromatography (GPC), ion chromatography, and LC/MS.Many analysis techniques use LCMS, which requires a front-end HPLC system to provide solvent delivery performance in the micro to semi-micro range and injectors with low sample carryover. Prominence is a network-ready HPLC system that meets the demands of today's advanced users. Prominence features the world's first Web control, fastest sample injection, and highest detection sensitivity performance…functions that surpass current HPLC technology.Prominence HPLC System Features: Reduced total analysis time - The SIL-20A Series autosamplers achieve unparalleled speed, with a sample injection movement of just 10 seconds. Extremely Low Sample Carryover - Multi-rinse mode achieves optimal rinsing for proteins and other sample components that are difficult to wash away. Hardware Expandability - Prominence allows configuration of the optimal analysis system for any application. Software Expandability - Range of software environments to apply Prominence to diverse fields Auto-purge functionality.
Prominence-i GPC System
World-class Small Multi Detection GPC System. A refractive index detector is generally used for GPC analysis of hydrophobic polymers. When trace-level additives that have UV absorption are contained in a polymer, both a UV detector and a photodiode array detector, which offers high-sensitivity detection, are used complementarily.By using the Prominence-i (PDA model), LabSolutions workstation, and LabSolutions GPC software (option), it is possible to simultaneously perform measurement of molecular weight distribution of a main component polymer using analysis results from the refractive index detector, and quantitative and qualitative analysis of trace-level additives using analysis results from the photodiode array detector.By incorporating a dual-temperature control function, which suppresses the effects of room temperature fluctuations, the RID-20A achieves excellent baseline stability and reproducibility. Moreover, the RID-20A significantly reduces stabilization time after being powered on. The stable baseline enables accurate analysis of molecular weight distribution. Shown below are a chromatogram and reproducibility of elution time results, over the course of five days, for an analysis of polystyrene containing additives.