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Analysis and Purification of Diastereomeric Steroid Saponins from the Trigonella foenum-graecum Seed Extract

16 Jul 2012

This application note reports the chromatographic resolution of a pair of 25 R/S spirostanol saponin diastereomers from Trigonella foenum-graecum seed extract using the Waters Method Station SFC System. The advantages of using SFC for spirostanol saponin analysis and purification are discussed.

Analytical Supercritical Fluid Chromatography Method Station II

Waters

Thar Instruments, makers of the TharSFC line of green chromatography Method Station system, is what most people describe as a methods development system for screening multiple compounds with multiple columns and multiple mobile phase compositions. The Analytical Supercritical Fluid Chromatography Method Station II features a smaller footprint despite a new larger Analytical–2–Prep Column Oven and expanded capacity high–speed Alias autosampler and optional Waters Photo Diode Array Detector. Additionally, the new ChromScope software package allows for 21 CFR Part 11 compliant environments as does our Dionex Chromeleon integration.

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Analysis and Purification of Diastereomeric Steroid Saponins from the Trigonella foenum-graecum Seed Extract