A Novel Approach to Detecting RNA Expression in Living Breast Cancer Cells
20 Jun 2013When studying the complexities of cancer biomarkers, the ability to monitor gene expression within live that have become cancerous, provides better insight than examining fixed or lysed tissues. However, monitoring RNA within intact cells can prove to be challenging with current techniques, which involve complex RNA isolation procedures that compromise cell viability. Further, amplification methods can create false positives or erroneously inflate differences. In this poster read about a probe-based detection method that allows for the detection of ErbB-2 and EGFR mRNA levels across live intact SKBR-3 breast cancer cells without the need for transfection reagents. The detection methodology is based upon an oligonucleotide gold nanoparticle conjugate capable of detecting intracellular levels of mRNA and miRNA in living cells. The probes enter the cell by means of the cell’s native endocytosis machinery.
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SmartFlare RNA Detection Probes
MerckSmartFlare Probes work by recognizing specific native RNA sequences within living cells, therefore there are many possible sequences that you could design to recognize your target of interest. Instead of lysed cells, switch to live cells. And while you’re at it, eliminate sample preparation and transfection steps all together. Live cell RNA detection is now possible, in a single incubation step using inert nanoparticle technology to specifically detect native RNA. And when you’re done, the probes exit the cells allowing you to perform downstream analyses with the same, unperturbed cells. Make the smart change!SmartFlare RNA Detection Probes are fueled by the NanoFlare technology developed by renowned chemist Dr. Chad Mirkin.What's Smart? Detect RNA expression in live cells for real time, physiologically relevant data Eliminate laborious, costly sample preparation Easy analysis on the fluorescent detection platform of your choice Nanoparticle based technology that allows cells to be used for downstream assays