Protein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Done in a Snap: Snap Tec ® makes the difference. Fast and safe sample handling with Eppendorf Conical Tubes SnapTec ® 50. Accelerate your workflow especially multistep protocols with easy single-handed operation and directly applicable in your laboratory environment.
Enzyme Immunoassay for the Quantitative determination of Protein L Ligand from medium containing immunoglobulin (Ig) or Ig-fragments. Accurate, robust results with a wider assay range, allowing for better quantification of the protein L contaminant.
High-throughtput titer quantification assays for antibodies, bispecifics and Fc fusion proteins
MultiVac™ Vacuum Manifolds have 3-port and 6-port options in stainless steel and aluminum. Compatible with all Rocker filter holders and mainstream disposable funnels from Sartorious, Pall, and Millipore.
Combined with pulsed amperometric detection (PAD), this anion-exchange column provides fast, reproducible separations with no need for derivatization. The 1 mm (standard/microbore) Dionex CarboPac PA1, 1 mm columns for Dual EGC contain the same resin as the proven 4 mm and 2 mm Dionex CarboPac PA1 columns, which are used for non-Dual EGC carbohydrate analysis.
The AxyPrep Mag PCR Clean-Up Kit is a unique paramagnetic bead-based post PCR clean-up kit designed for efficient removal of salts, primers, primer-dimers, dNTPs, and enzymes. Highly purified DNA is eluted with an optimized buffer so that it can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapter ligation, and…
Fast, reliable, and reproducible enrichment of low-abundant proteins from plasma and serum, based on novel ENRICH technology and in-StageTip (iST) technology.
Overcoming challenges to carry out successful filter integrity testing within a regulated environment
High-capacity filters suitable for most applications.
High-capacity filters capable of achieving an extremely high level of bioburden control.
Highly efficient miniaturized chromatography columns designed for rapid process development and high-throughput screening. Ready-to-use and pre-packed, these columns are perfectly suited for automated liquid handling systems, ensuring convenience and precision.
Rapid, automatable, and robust protein digestion workflow.
Lot certified oligonucleotide standards that allow you to benchmark column and LC system performance with confidence.
Atlantis Premier BEH Z-HILIC Columns provide an increase in polar analyte retention and offer a different selectivity when compared to other HILIC chemistries, both powerful attributes when performing HILIC methods development. Built using rugged ethylene bridged hybrid particles, these columns are pH stable from pH 2-10; the MaxPeak High Performance Surface hardware technology significantly reduces unwanted analyte/surface i…
Reagent kit that enables rapid lysis of yeast cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases Application Use Complete Lysis-Y to rapidly lyse cells of various yeast strains (e.g., Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris) in only 20 minutes at room temperature and simultaneously inhibit protease activity in the cell ly…
Reagent kit that enables rapid lysis of bacteria for highly efficient protein extraction and concurrent protection of extracted proteins against a multitude of proteases. Suitable for downstream purification using IMAC. Application Use Complete Lysis-B (2x), EDTA-free to rapidly lyse bacteria in only 10 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein extraction…
Reagent kit that enables rapid lysis of bacteria for highly efficient protein extraction and concurrent protection of extracted proteins against a multitude of proteases Application Use Complete Lysis-B (2x) to rapidly lyse bacteria in only 10 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein extraction and concurrent inhibition of a multitude of proteases, includ…
Reagent kit that enables rapid lysis of mammalian cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases. Suitable for downstream purification using IMAC. Application Use Complete Lysis-M, EDTA-free to rapidly lyse mammalian cells in only 5 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein e…
Reagent kit that enables rapid lysis of mammalian cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases Application Use Complete Lysis-M to rapidly lyse mammalian cells in only 5 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein extraction and concurrent inhibition of a multitude of proteas…
Reagent kit that enables rapid lysis of yeast cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases. Suitable for downstream purification using IMAC. Application Use Complete Lysis-Y, EDTA-free for the rapid cell lysis of various yeast strains (e.g., Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris) in only 20 minutes at room tempera…
The Steptavidin is produced by fermentation of S. avidinii in a minimal medium. The protein has a structure with four identical subunits, each containing a binding site for biotin. It is stable over a wide range of pH and decomposes only in the presence of SDS at temperatures above 60 °C. Steptavidin conjugated with an enzyme, i.e. Horseradish Peroxidase or Alkaline Phosphatase or a fluorochrome may be used to visualize biot…
The Empore™ High Performance Extraction Disk Plate differs in design from plates offered by other manufacturers and requires a specific vacuum manifold. Manifolds supplied by different plate manufacturers are specific to the design of their plates and cannot generally be used with plates from other manufacturers. In some cases, the use of spacers to raise/lower the height of a collection device inside the manifold may overco…
Replacement gaskets are available with the vacuum manifold assembly. The gasket can become worn over time and the seal between the plate and the manifold will be affected. This may result in poor vacuum pressure and negatively effect flow rates. The gasket should be checked regularly and replaced when it becomes worn.
Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. The AbSelect™ Purification Kit quickly removes these contaminants of antibody labelling. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up ideally is in a volume of 100ul to 0.5m…
