Immun-Blot Goat Anti-Mouse IgG (H+L)-AP Assay Kit #1706461
Bio-RadColorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays
Western blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays
2 ml, blotting-grade horseradish peroxidase secondary antibody conjugate
2 ml, blotting-grade horseradish peroxidase secondary antibody conjugate
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate
1 ml, blotting-grade horseradish peroxidase secondary antibody conjugate
Pkg of 1, lyophilized goat anti-mouse IgG conjugated with StarBright Blue 520 Fluorophore; reconstitute to 400 µl
Pkg of 1, lyophilized goat anti-mouse IgG conjugated with StarBright Blue 520 Fluorophore; reconstitute to 80 µl
Pkg of 1, lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 520 Fluorophore; reconstitute to 400 µl
Pkg of 1, lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 520 Fluorophore; reconstitute to 80 µl
The high titer of the blotting-grade antibody conjugates increase assay sensitivity. Greater working dilutions (1:3,000) decrease background and increase the signal-to-noise ratio of the assay.
EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.
Founded in 1954 Vilber are leaders in molecular imaging, having equipped more than 20,000 laboratories worldwide. Vilber Fusion blot imaging systems have been used by 3 Nobel prize winners. The latest generation of Fusion systems offer ‘better than film’ sensitivity with user upgradability for multi-colour fluorescence blotting.
The Invitrogen™ iBright™ CL750 Imaging System offers the core essential western blot and gel imaging functions and supports a smooth transition from darkroom and film-based detection methods to digital imaging.
Flexible imagers with automated features for sensitive, accurate chemiluminescence and fluorescence detection. To support your electronic data management, an optional GxP module is available, designed to meet increasing requirements of regulations like FDA 21 CFR part 11 and EU GMP Annex 11.
Excitable by the violet 405 nm laser and emitting at 515 nm, StarBright Violet 515 (SBV515) Dye is a perfect replacement for fluorophores that have emission maxima between 500 and 550 nm, such as Pacific Orange, Krome Orange, vioGreen, and Brilliant Violet 510, which can all be detected with a 525/50 filter (or similar) in your flow cytometry experiments. Minimal excitation by other lasers and a narrow emission profile make St…