Gene Expression and Molecular Cloning Products & Reviews

A significant advance in the technology of fabrication of micropipettes, optical fiber probes, and nanospray tips, is offered with the P-2000 micropipette puller. The P-2000 integrates a CO2 laser-based heat source with the technology derived from our extensive experience with conventional pullers. This system offers capabilities unmatched by other pullers. Key Features: Capable of pulling quartz, borosilicate and alumino…

This system incorporates features that make it the most technically advanced exponential decay electroporation system currently available. The generator utilizes the BTX Power Platform Technology design and novel digital user interface. The revolutionary Precision Pulse System provides the researcher unparalleled power in controlling the time constant. With the ability to deliver a maximum of 6000 A in the Low Voltage Mode, th…

GeneMapper® Software is a flexible genotyping software package that provides DNA sizing and quality allele calls for all Applied Biosystems electrophoresis-based genotyping systems. This software specializes in multiapplication functionality, including amplified fragment length polymorphism (AFLP®), loss of heterozygosity (LOH), microsatellite, and SNP genotyping analysis. GeneMapper® Software can help users increase data proc…

Reliable Transfection for Common Cell Lines

High productivityVersatile: efficient in both HEK 293 & CHO synthetic mediaComplies with new regulatory requirements for large scale production

The Actin Polymerization Biochem Kit™ is based on the enhanced fluorescence of pyrene conjugated actin that occurs during polymerization. The enhanced fluorescence that occurs when pyrene G-actin (monomer) forms pyrene F-actin can be measured in a fluorimeter to follow polymerization over time. Also, by using preformed pyrene F-actin, it is possible to follow depolymerization. Both cell/tissue extracts and purified proteins ca…

Oligonucleotide GMP manufacturing for human therapeutics

Blood Culture Yeast Verification Kit (RUO), Blood Culture GP Verification Kit (RUO) and Blood Culture GN Verification Kit (RUO) are designed to verify the performance of molecular testing processes that detect the presence of nucleic acids from various yeasts or bacteria. Supplied in single-use vials, these kits can also be used to train and evaluate operator proficiency.

MDx-Chex for Blood Culture Yeast (RUO), MDx-Chex for Blood Culture GP (RUO) and MDx-Chex for Blood Culture GN (RUO) are full-process molecular quality controls designed for comprehensive molecular assay workflow validation.

Specially formulated for high yields of canonical (GAUC) in vitro transcribed RNA. Produce up to 90 µg of RNA from 1 µg of template in 2 hours. Easily scale up reactions to produce milligram amounts of RNA.

Generate canonical (GAUC) mRNA with greatly reduced double-stranded RNA (dsRNA) content. Easily remove dsRNA without the need for chromatographic methods like HPLC. Produce large amounts of mRNA with virtually 100% transcript capping. Reduce the innate immunogenic response to mRNA in cells.

Amplify micrograms of circular DNA from as little as 5 pg of input. Directly amplify from purified plasmid, glycerol stocks, liquid media cultures, or colonies on agar plates. Accurate amplification, with an error rate of ~1 in 106-107 bases.

Build Cap 0 caps with nearly 100% efficiency. Use simultaneously with ScriptCap™ 2'-O-Methyltransferase to produce Cap 1 caps.

Co-transcriptionally cap in vitro transcribed RNA with Anti-Reverse cap analog (ARCA). Produce up to 60 µg of capped RNA from 1 µg of template in just 30 minutes. Optimized for maximum transcript capping and yield.

All-in-one kit combining the benefits of high-yield transcription with post-transcriptional capping and tailing. Produce large amounts of mRNA with virtually 100% transcript capping.

Specially formulated for high yields of canonical (GAUC) in vitro transcribed RNA. Produce up to 150 µg of RNA from 1 µg of template in 2 hours. Easily scale up reactions to produce milligram amounts of RNA.

Generate N1-methyl-pseudouridine-containing mRNA (N1meΨ-mRNA) with greatly reduced double-stranded RNA (dsRNA) content. Easily remove dsRNA without the need for chromatographic methods like HPLC. Produce large amounts of N1meΨ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells.

Remove cap structures (decapping) from various RNAs. Substitute unit for unit into any prexisting protocol for Tobacco Acid Pyrophosphatase (TAP).

Build Cap 0 or Cap 1 caps with nearly 100% efficiency. Provides all reagents for capping in vitro transcribed RNA.

Prepare Cap 1-RNA from any source of Cap 0-RNA. Use simultaneously with ScriptCap™ m7G Capping System to produce virtually 100% Cap 1-capped RNA in a single reaction.

Generate pseudouridine-containing mRNA (Ψ-mRNA) with greatly reduced double-stranded RNA (dsRNA) content. Easily remove dsRNA without the need for chromatographic methods like HPLC. Produce large amounts of Ψ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells.

Specially formulated for maximum yields of canonical (GAUC) in vitro transcribed RNA. Produce up to 180 µg of RNA from 1 µg of template in just 30 minutes. Easily scale up reactions to produce milligram amounts of RNA.

Optimized for high-yield synthesis of 5-methyl-cytidine- pseudouridine-containing, in vitro transcribed RNA (5mCΨ-RNA). Produce up to 100 µg of 5mCΨ-RNA from 1 µg of template in 2 hours. Easily scale up to produce milligram amounts of 5mCΨ-RNA. Induce lower innate immune responses in mammalian cells by using 5mCΨ-mRNAs.

Quantitative mRNA poly(A) tail length using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective benchtop method for QC as an alternative to mass spec analysis. Assess tail lengths above the limits of HPLC and LC-MS based methods.

Quantitate mRNA capping efficiency (percent capped RNA content) of XBG 5’ UTR-containing mRNA using standard polyacrylamide gel electrophoresis (PAGE). XBG 5’ UTR Targeting Oligonucleotide and XBG 5’ UTR Control Mix included in kit. Easy, cost-effective, benchtop method useful for QC as an alternative to HPLC and LC-MS analysis.