Genome Analysis Products & Reviews

Thermo Scientific Exonuclease I digests single-stranded DNA 3' to 5' (requires Mg2+ and a free 3'-OH). The nuclease is inactivated by heating at 80°C for 15 minutes.

Thermo Scientific Shrimp Nuclease is an endonuclease that cleaves phosphodiester linkages in DNA to yield di- and oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. The enzyme has a high preference for double-stranded DNA, and can be used to selectively degrade dsDNA, leaving ssDNA and RNA intact. The enzyme is inactivated after 15 minutes at 65 °C.

Thermo Scientific RNase A (ribonuclease A) is supplied as a chromatographically pure freeze-dried powder, salt-free for easy reconstitution in most buffers.

Thermo Scientific Ribonuclease H (RNase H) is an endonuclease that degrades the RNA portion of DNA-RNA hybrids. It hydrolyses phosphodiester bonds of RNA in RNA:DNA heteroduplexes. It produces products with 3’-OH and 5’-phosphate. RNase H will not degrade single or double-stranded DNA or RNA.

Thermo Scientific Shrimp Alkaline Phosphatase (SAP) catalyzes the dephosphorylation of 5´ phosphates from DNA and RNA and is completely and irreversibly inactivated by heating at 65°C for 15 minutes. SAP is active on 5´ overhangs, 5´ recessed and blunt ends.

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indoyl-ß-D-galactopyranoside) is a chromogenic substrate used to identify recombinant plasmids. In the presence of X-Gal and IPTG, lac+ bacterial colonies appear blue, whereas recombinant colonies appear white.

Source: UCSC Build: HG18, NCBI 36 Probe Length: 60mer Median Probe Spacing: 1169bp Platform: HD2 Probes per Array: 2,100,000 Feature Size: 13μm x 13μm Array Dimensions: 62mm x 14mm Overall Slide Dimensions: 1" x 3" (25 x 75mm) Recommended Storage: Store arrays desiccated at room temperature.

Kit includes OpenArray® Endpoint Analysis Software CD and user’s manual. Compatible with the NT Imager or NT Cycler.

Customer-owned TaqMan® assays reformatted on OpenArray® plates. Plates must be ordered in multiples of 10. Minimum order is 10 plates. Kit includes: Cases, Immersion Fluid, Case Sealing Glue and Plate-File CD.

OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for ADME and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray plate, Real…

OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Apoptosis and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray plate,…

OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Cancer and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray plate, Re…

OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Cardiovascular Disease and related genes reformatted onto an OpenArray plate. Kit includes: Ope…

OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Inflammation and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray pla…

Real-Time qPCR assays for Signal Transduction and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray plate, Real-Time qPCR Case, Immersion Fluid, Frame Adhesive and Plate-File CD.

STN is an online database service that provides global access to published research, journal literature, patents, structures, sequences, properties, and other data. 

The Applied Biosystems SNPlex Genotyping System enables the simultaneous genotyping of up to 48 SNPs against a single biological sample. This system is ideal for fine mapping and candidate gene analysis, population stratification and microarray replication studies.

Axygen X-Gal IPTG spray is a convenient ready-to-use solution of optimal quantities of IPTG, X-Gal and stabilizers presented in a spray bottle for easy, accurate and consistent screening of Blue/White colonies. Advantages of Axygen X-Gal-IPTG Spray include:• Easy, accurate and convenient. • Sprays deliver optimal concentration and volume consistently for consistent and accurate results. • No need to weigh X-Gal and IPTG and…

The  AxyPrep™-96 Plasmid Purification Kit represents a true high-throughput system for the purification of plasmid or cosmid DNA in a 96-well format. The AxyPrep™-96 Plasmid Purification Kit is designed to produce up to 20 mg of highly purified plasmid or cosmid DNA (per well) from up to 1.3 ml of bacterial culture in 96-well format. With the AxyPrep™-96 Plasmid Purification Kit two plates rapidly and efficiently process the…

AxyPrep™ Easy-96 Plasmid Purification Kit employs a modified alkaline lysis method in combination with an optimized 96-well lysate filtration plate and an alcohol precipitation step to purify plasmid and cosmid DNA from bacterial cultures. The AxyPrep™ Easy-96 Plasmid Purification Kit is designed to process 1.3 ml aliquots of bacterial culture, grown in a 96-well format. Plasmid or cosmid DNA prepared by the Axyprep Easy-96…

This kit is based upon a modified SDS-alkaline lysis of bacterial cells in combination with lysate filtration and selective binding of the plasmid DNA to special AxyPrep purification columns. The yield of plasmid for this kit is up to 100 mg. This kits can also be used to isolate large constructs, such as BAC, PAC and P1. The protocols provide a simple, rapid and efficient method for the isolation of highly purified plasmid…

AxyPrep™ Body Fluid Viral DNA/RNA Miniprep Kit provides a rapid and highly reproducible way to purify viral nucleic acid from 250 ml of body fluid, including plasma, serum, ascites, cerebrospinal fluid, urine, cell culture supernatant, etc. With the AxyPrep™ Miniprep Kit, during lysis, proteins and PCR inhibitors are removed by denaturation and precipitation. Viral nucleic acids remain soluble in the supernatant and are puri…

The AxyPrep Multisource Total RNA Miniprep Kit from Axygen allows the purification of total cellular RNA from a diverse array of starting materials. The AxyPrep Multisource Total RNA Miniprep Kit represents a new approach to RNA purification, and is designed to eliminate the problems of column clogging and incomplete purification associated with other products. The optimized protocol of the AxyPrep Multisource Total RNA Mini…

The AxyPrep Blood Total RNA Miniprep Kit is designed to process up to 400 µl of anticoagulated whole blood per prep. RBCs are removed using a special lysis buffer. Following WBC lysis, genomic DNA and protein are removed via precipitation, which significantly improves the yield and purity of the total RNA sample. The carryover of genomic DNA is greatly reduced and the opportunity for column clogging is eliminated, ensuring o…

AxyPrep™ Multisource Total RNA Midiprep Kit from Axygen Scientific Format of the AxyPrep™ Multisource Total RNA Midiprep Kit from Axygen: • Midi column AxyPrep™ Multisource Total RNA Midiprep Kit Starting Materials: • Animal tissues • Plant tissue • Cultured cells • Bacteria • Yeast • Filamentous fungi Features of the Axygen AxyPrep™ Multisource Total RNA Midiprep Kit: • Rapid spin and vacuum protocols • No alcohol precipitat…