PCR Reagents Products & Reviews

The universal reverse primer is designed for use with the Human U6 forward primer provided in our High-Specificity miRNA QRT-PCR Detection Kit for a complete, validated assay requiring no upfront design work. The Kit delivers sensitive and reproducible detection of miRNA with high specificity.

Our SideStep Lysis and Stabilization Buffer ensures accurate gene expression analysis by eliminating sample loss and degradation. The proprietary buffer lyses cells and immediately stabilizes the released nucleic acid for months. Several formats are available to enhance research versatility.

Taq Extender PCR additive improves the reliability and yield of conventional Taq-based PCR amplifications. Taq Extender additive increases the efficiency of Taq DNA polymerase extension reactions during each cycle of PCR by reducing mismatch pausing, resulting in a greater percentage of completed extension reactions.

The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reactions buffers contain the optimal magnesium concentration for efficient PCR.

Sensitive Quantitation of Single-Stranded DNARequires less DNA than spectrophotometry

A patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR.

An optimized photoactivation device designed for photolysis of PMAxx™- and PMA- treated samples in viability PCR.

EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

A high-performance dye-based qPCR master mix containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. With low or high ROX.

A high-performance dye-based qPCR master mix containing EvaGreen®, Cheetah™ HotStart Taq Polymerase, and the Forget-Me-Not™ 2-color tracking system.

A high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.

Modifications that enhance the performance of oligonucleotides

IGT® Adapter & UDI Primers is a series of adapter & unique dual-indexed primer kits used for NGS library construction of dsDNA, ssDNA and methylation DNA. iGeneTech provides 384 combinations of different UDI primers for high-throughput sequencing in a single lane, which reduces the false-positive rate caused by the index contaminations due to index hopping. For MultipSeq® library construction and low-frequency mutation tests,…

PCR Water is a molecular biology grade product suitable for sample dilution, and use as an ultra-pure diluent for PCRs and other molecular biology reactions.

Easy-to-use, one-component reagent for internal labeling of oligonucleotide probes to be detected on the LightCycler® and LightTyper Instruments. Application The SimpleProbe 519 Labeling Reagent is used for the internal labeling of oligonucleotide probes that are to be detected at  519 nm, for example, when used as mutation detection probes on the LightCycler® and LightTyper Systems following the SimpleProbe probe principle.…

Precision Blue™ Real-Time PCR Dye, 55 µl (500 x 20 µl reactions)

High-quality, molecular-grade dNTPs. Kapa offers premium grade dNTPs for PCR. KAPA dNTP Mix contains dATP, dCTP, dGTP, and dTTP supplied at a concentration of 10 mM each.   Applications: Standard PCR qPCR cDNA synthesis DNA sequencing DNA labeling Microarrays

PCR oligonucleotide primer sets consisting of 16  primers  for amplification and 16 primers  for cloning of human IgG and IgM heavy and light chain variable domain coding regions for generating universal, patient or antibody-specific scFv phage-display libraries. 

PCR oligonucleotide primer sets consisting of 25 primers for amplification and 25 primers for cloning of mouse IgG heavy and light chain variable domain coding regions for generating universal or antibody-specific scFv phage-display libraries.

Universal 16S rDNA real-time PCR assay for sensitive detection of bacterial DNA

98-100%

Universal 18S rDNA real-time PCR assay for sensitive detection of fungal DNA

High purity, well characterized trehalose in powder or solution

HiScript III U+ One Step qRT-PCR Probe Kit is specially designed for qPCR that directly use RNA (e.g. virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. 

This ready-to-use nucleotide mix is a premixed solution of multiple PCR-grade nucleotide sodium salts in water: dATP, dGTP, dCTP, and dTTP (each at a concentration of 10 mM). This mix is optimized for use in all types of amplification reactions and primer-extension reactions. PCR RT-PCR cDNA synthesis Primer extension Benefits Improve yield and performance Choose a convenient ready-to-use mix of all 4 nucleoti…