PCR and Thermal Cycling Products & Reviews

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Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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MegaMix Emerald - qPCR Dye Based

Microzone

Ideal for quantification of any DNA template (gDNA, cDNA, viral DNA), amplifies low copy number samples with ease. Utilises our intercalating fluorescent dye, MicroGREEN, that binds to double stranded DNA, making it the perfect choice for qPCR & melt curve analysis.

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microGREEN Dye qPCR

Microzone

Swift, cost-effective qPCR detection. Compatible with a wide range of qPCR instruments and mastermixes. Heightened sensitivity and outstanding reproducibility due to high fluorescence. A robust, thermally stable dye endures cycling and freeze-thaw cycles exceptionally well. 

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MegaMix Gold RT-PCR Mastermix

Microzone

The MegaMix Gold One-step RT-PCR kit contains all the components needed to perform RT-PCR swiftly and reliably. The kit contains all the components needed to perform RT-PCR swiftly and reliably. The kit ensures a highly specific and sensitive amplification, removing background and primer dimer formation. MegaMix Gold boasts excellent accuracy and produces A-tailed products suitable for ligating into TA cloning vectors.

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Countable PCR system bundle

Countable Labs

Countable PCR is the only PCR-based method that directly counts your targets, giving you unprecedented and highly sensitive insights into your samples. Don't be limited by qPCR, dPCR, or even NGS. Find even the rarest targets when you experience single-molecule precision and ultra-high sensitivity in a familiar PCR workflow. Plus – quickly develop a new multiplex assay in 3 days.

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Universal Multiplex Kit

Countable Labs

Universal Multiplexing from Countable Labs enables you to design and run up to 4-plex multiplex experiments within three days. Use with your Countable PCR system to reduce the time and cost it takes to develop multiplex experiments.

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Countable Matrix Reagents Kit

Countable Labs

The Countable Matrix is the key to getting direct single-molecule counts of your multiplex targets with Countable PCR. This kit enables you to prepare one-tube reactions with over 30 million compartments, arranging your targets spatially in a clear 3D gel matrix and expanding the sensitivity and precision of your PCR assays

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Air-Dryable Probe Mix

PCR Biosystems

Air-Dryable Probe Mix is a versatile, air-dryable 4x qPCR reagent mix for ultra-sensitive detection of DNA sequences. The mix is glycerol-free and contains hot start Taq polymerase, dNTPs, Mg and a blend of excipients to ensure reliable air-drying, without loss of activity.

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Clara® Inhibitor-Tolerant Probe Mix

PCR Biosystems

Unlock the power of precise qPCR analysis with Clara® Inhibitor-Tolerant Probe Mix. This broad-spectrum, inhibitor-tolerant qPCR mix is designed to overcome challenging laboratory, environmental, and clinical sample inhibitors, ensuring reliable results and streamlined workflows for your research.

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Clara® Probe 1-Step Purple Mix

PCR Biosystems

Clara® Probe 1-Step Purple Mix offers clear and consistent results every time, with an easy-to-see, inert, purple dye for sample visualisation. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.

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Air-Dryable Inhibitor-Tolerant Probe 1-Step Mix

PCR Biosystems

Air-Dryable Inhibitor-Tolerant Probe 1-Step Mix is a glycerol-free 4x RT-qPCR mix that combines our novel inhibitor-tolerant chemistry alongside our cutting-edge air-dryable technology. Now you can benefit from dried, room temperature stable qPCR assays capable of highly-sensitive RNA & DNA detection on the most challenging, inhibitor-rich sample types.

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Air-Dryable Inhibitor-Tolerant Probe Mix

PCR Biosystems

Air-Dryable Inhibitor-Tolerant Probe Mix is a glycerol-free 4x qPCR mix that combines our novel inhibitor-tolerant chemistry alongside our cutting-edge air-dryable technology. Now you can benefit from dried, room-temperature stable qPCR assays capable of highly-sensitive DNA detection on the most challenging, inhibitor-rich sample types.

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Clara® Inhibitor-Tolerant Probe 1-Step Mix

PCR Biosystems

Unlock the power of precise RT-qPCR analysis with Clara® Inhibitor-Tolerant Probe 1-Step Mix. This broad-spectrum, inhibitor-tolerant RT-qPCR mix is designed to overcome challenging laboratory, environmental, and clinical sample inhibitors, ensuring reliable results and streamlined workflows for your research.

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PCRBIO Taq Mix

PCR Biosystems

PCRBIO Taq Mix is an affordable, versatile and robust 2x mix for all your everyday PCR applications including genotyping, screening and library construction. An enhanced 12-step polymerase purification strategy together with an optimised buffer system enable PCRBIO Taq Mix to amplify with the highest speed, yield and specificity.

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PCRBIO HS Taq Mix

PCR Biosystems

PCRBIO HS Taq Mix is an advanced antibody-mediated hot start DNA polymerase mix designed for fast, highly specific and sensitive PCR. Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.

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PCRBIO HS Taq DNA Polymerase

PCR Biosystems

PCRBIO HS Taq DNA Polymerase is an advanced antibody-mediated hot start DNA polymerase designed for fast, highly specific and sensitive PCR. Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.

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UltraScript® 2.0 Reverse Transcriptase

PCR Biosystems

UltraScript® 2.0 Reverse Transcriptase is a highly thermostable reverse transcriptase designed for cDNA synthesis of the most challenging RNA sample types. This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55 °C, giving improved specificity, higher cDNA yields and more full length cDNA product.

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qPCRBIO SyGreen® Blue Mix

PCR Biosystems

qPCRBIO SyGreen® Blue Mix uses a non-reactive blue dye for easy sample visualisation during reaction setup, allowing fast, highly sensitive and reproducible real-time PCR with more precision and accuracy than ever. This easy-to-see mix is particularly useful in reducing errors when loading small volume reactions or using white plates.

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VeriFi® Mix

PCR Biosystems

VeriFi® Mix is a versatile and robust high fidelity 2x ready-mix engineered for all PCR applications where greater sequence accuracy is required. Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets. 

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VeriFi® Hot Start Mix

PCR Biosystems

VeriFi® Hot Start Mix is a versatile and robust 2x proofreading ready-mix with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

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