Kit for the isolation of total RNA from mammalian whole blood, blood cells, and cultured cells using the MagNA Pure LC Instrument For general laboratory use. The MagNA Pure LC RNA Isolation Kit - High Performance is designed to isolate high-purity total RNA from mammalian whole blood, blood cells, or cultured cells using the MagNA Pure LC Instrument. The purified RNA can be used directly in RT-PCR on the LightCycler® Carousel-…
Kit for the isolation of mRNA from mammalian whole blood, blood cells, or cultured cells using the MagNA Pure LC Instrument For general laboratory use. The MagNA Pure LC mRNA Isolation Kit I (Blood, Blood Cells) is specifically designed for use with the MagNA Pure LC Instrument to isolate high-quality, undegraded mRNA from mammalian whole blood, blood cells, or cultured cells. The purified mRNA can be used directly in RT-PCR…
AmpliTaq® DNA Polymerase is a 94 kDa ultra-pure, gelatin-free, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus Taq DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the polymerase chain reaction (PCR) process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lo…
Product Overview: The MILLIPLEX® Human Stem Cell Pluripotency Magnetic Bead Kit 2 (Cat. No. 48-620MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes in cell lysates: E-Cadherin, EpCam, KLF4, LIN-28, and the glycostructures TRA-1-60, TRA-1-81 and SSEA1. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has suffic…
The NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents in…
A broad spectrum, low toxicity DNA transfection reagent optimized for 96-well plate applicationsTransIT®-Express Transfection Reagent is specifically developed for high-throughput transfection of plasmid DNA into mammalian cells. TransIT-Express Reagent provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use and repro…
A nontoxic, "Quick Recovery" In Vivo delivery solution that delivers DNA or siRNA to the livers of laboratory mice using methods including hydrodynamic tail vein injection.The TransIT-QR (Quick Recovery) Hydrodynamic Delivery Solution is designed specifically for the safe and efficient delivery of nucleic acids into laboratory mice using the hydrodynamic tail vein injection procedure. This formulation is optimized for efficien…
Corning® Collagen I, bovine, 30mg, is used either as a thin layer on tissue-culture surfaces to enhance cell attachment and proliferation, or as a gel to promote expression of cell-specific morphology and function. (Same as Cat. #40231)SourceBovine dermisQualityPurity > 95% by SDS-PAGEShown to promote attachment and spreading of HT-1080 human fibrosarcoma cellsTested for formation of a firm gel by exposure to ammonia vapors fr…
The enzyme dNTPack comprises the Pwo SuperYield DNA Polymerase and PCR-Grade Nucleotides in a ready-to-use solution.Pwo SuperYield DNA Polymerase is ideal for high-fidelity amplification of DNA up to 3 kb. Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with a newly optimized buffer system. This new buffer system enhances the enzymatic properties of the polymerase, resulting in higher yields of…
Thermo Scientific ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments. ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity. The master mixes incorporate an inert blue dye to enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.…
Ion Channel Cell Lines - Validated in Performance, Accuracy and Quality In cooperation with Cytocentrics AG CCS has developed a set of recombinant cell lines stably expressing ion channels of high pharmacological relevance. The cell lines have been generated by stable transfection of HEK293 or CHO-K1 cells and subsequent clonal selection. They have been characterized in manual and automatic patch clamp assays regarding their…
Phusion™ RT-PCR Kit is a complete two-step kit designed for high-fidelity RT-PCR. The kit contains the full set of reagents required for performing cDNA synthesis and PCR in two steps. In the first step, a variety of RNA templates and all priming options may be used for efficient synthesis of cDNA with M-MuLV Reverse Transcriptase.In the second step, Phusion Hot Start High-Fidelity DNA Polymerase is used for cDNA amplification…
Thermo Scientific Verso 1-step RT-qPCR Kits for probe chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment…
NEBNext® dsDNA Fragmentase® generates dsDNA breaks in a time-dependent manner to yield 100–800 bp DNA fragments depending on reaction time. NEBNext dsDNA Fragmentase contains two enzymes, one randomly generates nicks on dsDNA and the other recognizes the nicked site and cuts the opposite DNA strand across from the nick, producing dsDNA breaks. The resulting DNA fragments contain short overhangs, 5´-phosphates, and 3´-hydroxyl…
The PureYield™ Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA. The system provides a rapid method for purification of up to 15μg of plasmid DNA from 600μl to 3ml of bacteria culture. Plasmid DNA can be purified in as little as 10 minutes. The PureYield™ Plasmid Miniprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxi…
Three kits to cover a wide range of cAMP concentrations.After the introduction of IP-One, the first HTS immunoassay that can easily detect inositol(1)phosphate (IP1), a stable second messenger which tightly correlates with Gq-coupled receptor activity, the optimization of cAMP assays with the new HTRF® acceptor d2 offers a complete GPCR platform.The upgraded cAMP dynamic 2 and cAMP femto 2 assays, along with the new cAMP HiRa…
The NEBNext® DNA Library Prep Master Mix Set for 454™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of single stranded DNA for use in high density hybridization arrays or for genomic subtraction hybridization methods. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation o…
The NEBNext® Fast DNA Library Prep Set for Ion Torrent™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing on a Ion Torrent PGM™. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. T…
The GS Junior Titanium emPCR Kit (Lib-A) provides reagents for the amplification of an Amplicon library from a single effective (i.e., functionally clonal) bead-bound copy of the DNA template to tens of millions of copies per bead, amenable to sequencing with the GS Junior Instrument using the appropriate GS Junior Titanium kits. One of the main advantages of the emPCR Amplification process is that, while the entire procedure…
The GS Junior Titanium emPCR Kit (Lib-L) provides reagents for the amplification of a Shotgun, Paired End or cDNA library from a single effective (i.e., functionally clonal) bead-bound copy of the DNA template to tens of millions of copies per bead, amenable to sequencing with the GS Junior Instrument using the appropriate GS Junior Titanium kits. One of the main advantages of the emPCR Amplification process is that, while the…
A high-quality RNA sequencing service designed to deliver cost-effective and rapid access to meaningful transcriptomics results. The Genefficiency™ RNA-Seq Service from OGT provides a new level of assay customisation to ensure each experiment is fully optimised to suit your specific study objectives. At OGT we understand the time and effort it takes to generate good quality RNA and how precious your samples are. Whether you a…
Mouse ROSA26 safe harbor gene knock-in kit and clones. ROSA26 (also known as ROSAβgeo26 locus) in mice genome is first found in chromosome 6 in one particular strain of mice-named ROSAβgeo26-expressed β galactosidase from a randomly inserted transgene at high levels uniformly in nearly all tissues examined. This locus expresses one coding transcript and two noncoding transcripts, and only the non-coding transcripts are disrup…
Viruses, such as SARS-CoV-2 (COVID-19), can cause serious health issues and play an important role in molecular biology and biomedical research. Having the right tools is critical for scientific discovery for COVID-19, including the efficient isolation of viral DNA/RNA with high purity and integrity, which is often a challenge. The Axygen AxyPrep MAG Viral Nucleic Acid Purification Kit utilizes a unique paramagnetic beads-base…
Viruses, such as SARS-CoV-2 (COVID-19), can cause serious health issues and play an important role in molecular biology and biomedical research. Having the right tools is critical for scientific discovery for COVID-19, including the efficient isolation of viral DNA/RNA with high purity and integrity, which is often a challenge. The Axygen AxyPrep MAG Viral Nucleic Acid Purification Kit utilizes a unique paramagnetic beads-base…
Assessing the genome-wide chromatin accessibility from individual cells provides unique insights of gene expression regulation for a number of fields such as development, oncology, and neurology. Bio-Rad’s SureCell ATAC-seq provides a fast, robust, and sensitive analysis of the epigenome at single-cell resolution. This method is powered by the combination of both Bio-Rad’s droplet-based microfluidic and a hyperactive Tn5 t…
