Real-Time PCR (qPCR) Mastermixes and Kits Products & Reviews

High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. The MeltDoctor™ HRM Master Mix contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed…

The SYBR® Green PCR Master Mix, optimized for real-time PCR analysis, conveniently combines SYBR Green 1 Dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer components in easy-to-use premix vials. SYBR® Green Master Mix has a new look! The new pouch design eliminates excess packaging materials, resulting in 72% less material than the carton with platform & label. The master mix inside…

Formulated with diagnostic kit developers in mind, RapiDxFire™ qPCR 5X Master Mix glycerol free (GF) can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation for use in point of care (POC) devices. Provided at a 5X concentration without passive reference dye, this flexible master mix allows more room…

The Yale 2019-nCoV SalivaDirect™ ValuPanel Reagents™ include FAM-BHQ and Quasar™ 670-BHQ probes and primers delivered in individual tubes in dried format.

Designed with input from top molecular diagnostic assay manufacturers, the RapiDxFire Lyo-Flex 1-Step RT-qPCR 5x Master Mix supports the development of diagnostic devices and assays that meet healthcare provider and consumer demand for fast, accurate results. With consistent results in a liquid state at room temp or in a lyophilised state, this master mix will help you achieve predictable performance in unpredictable conditio…

2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference d

2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 200 x 20 µl reactions

1 ml (1 x 1 ml vials), contains antibody-mediated hot-start Sso7d-based DNA polymerase (patented), dNTPs, salts, enhancers, stabilizers, for 50 x 50 µl reactions

5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX Normalization Dyes, for 500 x 20 µl reactions

5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX Normalization Dyes, for 500 x 20 µl reactions

10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 1,000 x 20 µl reactions

5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 500 x 20 µl reactions

10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 1,000 x 20 µl reactions

20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 2,000 x 20 µl reactions

25 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl, EvaGreen dye, stabilizers, for 2,500 x 20 µl reactions

50 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl, EvaGreen dye, stabilizers, for 5,000 x 20 µl reactions

100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for most templates

50 x 50 µl reactions, contains iScript Advanced Reverse Transcriptase, 2x PreAmp Mix (dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components), reaction booster, DNase, DNase buffer, nuclease-free water

Lentiviruses are commonly used as viral vectors in cell and gene therapy production, delivering and steadily integrating desired genes into the genome of the host cell. However, they may also present health hazards to humans due to integration-mediated transformation and the creation of a RCL that can infect nontarget cells. While the current regulatory guidelines recommend a cell culture–based method for RCL testing, it is cu…

Adeno-associated virus (AAV) is commonly used as a viral vector in cell and gene therapy production, but there is a risk of it becoming a replication competent virus (RCV) during the production process, especially in the presence of a helper virus, which can raise safety concerns. While the guidelines recommend a cell culture–based method for RCAAV testing, it is cumbersome and takes 30–45 days from sample to results. Verichec…

Brilliant II qPCR and qRT-PCR reagents maintain the high quality of our original Brilliant product line. Now has greater detection sensitivity, ensuring reproducible quantification even at low target concentrations. Adding ROX reference dye to the master mix streamlines the reaction setup process.

Brilliant II qPCR and qRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations. For faster experiments with earlier Ct's and greater reproducibility, Agilent recommends Brilliant III Ultrafast Master Mixes.

Brilliant II SYBR qPCR and QRT-PCR reagents provide improved sensitivity and detection while ensuring reproducible quantification at low target concentrations. Compared to other commercially available qPCR reagents, Brilliant II SYBR qPCR and QRT-PCR reagents have earlier threshold cycle (Ct) detection at high and low template concentrations.

Brilliant II SYBR® QPCR and QRT-PCR Master Mix Kits with High/Low Rox offer superior sensitivity for improved quantification and reproducibility. Brilliant II kits also show more consistent performance across a wide range of template concentrations and different targets.  New formulations available with low and high concentrations of ROX reference dye  Improved performance with earlier Ct detection across a wide dynamic ran…

Brilliant II QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations. When compared to other commercially available QPCR reagents, we observe earlier threshold cycle (Ct) detection at high and low template concentrations as well as improved reaction efficiency and replicate reproducibility across the entire range of template concentr…