Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
A broad spectrum, low toxicity, DNA transfection reagentTransIT®-LT1 Transfection Reagent is a broad spectrum reagent that provides high efficiency plasmid DNA delivery in many mammalian cell types including primary cells. TransIT-LT1 is a low toxicity, serum-compatible transfection reagent that eliminates the need for any culture medium change.TransIT-LT1 is suitable for both transient and stable transfection and can be used…
A high performance, animal-free, broad spectrum DNA transfection reagentTransIT®-2020 Transfection Reagent is a high-performance, animal-origin free, broad spectrum reagent that provides exceptional transfection of plasmid DNA into mammalian cells. TransIT-2020 affords high levels of gene expression in typically hard-to-transfect cell types, including primary cells and is suitable for both transient and stable transfection.Tra…
A high efficiency DNA transfection reagent optimized for Jurkat and other hard-to-transfect cell typesTransIT®-Jurkat Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in Jurkat cells and cell types of associated lineage.TransIT-Jurkat reagent also works well for additional cell lines of hematopoietic origin such as THP-1(human acute monocytic leukemia cell line) and R…
A high efficiency, low toxicity, DNA transfection reagent optimized for prostate cell typesTransIT®-Prostate Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in Prostate cell types. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use and reproducibil…
A high efficiency, low toxicity, DNA transfection reagent optimized for various neural cell typesTransIT-Neural® Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in various neural cell types. TransIT-Neural Reagent provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simpli…
A high efficiency, low toxicity, DNA transfection reagent optimized for CHO cell typesTransIT®-CHO Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in CHO cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use…
A high efficiency, low toxicity, DNA transfection reagent optimized for 3T3 cell typesTransIT®-3T3 Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in NIH 3T3 cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of…
A high efficiency, low toxicity, DNA transfection reagent optimized for COS cell typesTransIT®-COS Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in COS cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use…
A high efficiency, low toxicity, DNA transfection reagent optimized for keratinocyte cellsTransIT®-Keratinocyte Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in keratinocyte cell types such as NIKS (Near-Diploid Immortalized Keratinocytes) and primary keratinocytes. TransIT-Keratinocyte Reagent provides all the attributes of the trusted TransIT series of transfecti…
A high efficiency, low toxicity, DNA transfection reagent optimized for HeLa cellsTransIT-HeLaMONSTER® Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in HeLa cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity o…
Superior DNA transfection of breast cancer cell typesTransIT-BrCa Transfection Reagent is the first dedicated breast cancer cell line specific reagent available to researchers today. While it delivers efficiently to many breast cancer cell types, it also maintains low cellular toxicity resulting in reduced experimental biases. TransIT®-BrCa Transfection Reagent is specifically optimized to provide exceptional transfection effi…
A nontoxic, "Quick Recovery" In Vivo delivery solution that delivers DNA or siRNA to the livers of laboratory mice using methods including hydrodynamic tail vein injection.The TransIT-QR (Quick Recovery) Hydrodynamic Delivery Solution is designed specifically for the safe and efficient delivery of nucleic acids into laboratory mice using the hydrodynamic tail vein injection procedure. This formulation is optimized for efficien…
An "Enhanced Expression" In Vivo delivery solution that delivers DNA to the livers of laboratory mice using methods including hydrodynamic tail vein injection.The TransIT-EE (Enhanced Expression) Hydrodynamic Delivery Starter Kit is designed specifically for the delivery of DNA expression vectors into live laboratory mice using the hydrodynamic tail vein injection procedure. This formulation is designed to produce high-level g…
A high efficiency, low toxicity, large RNA transfection reagent for mammalian cells.TransIT®-mRNA Transfection Kit is designed to transfect RNA into a broad range of cell types with minimal cellular toxicity. RNA delivery avoids transcriptional regulation effects by directly delivering the RNA to the cytoplasm for expression. The TransIT-mRNA Transfection Kit can be used to deliver a variety of RNA molecules including mRNAs an…
A high efficiency, low toxicity, transfection reagent optimized for oligonucleotide delivery into a wide range of cell typesTransIT®-Oligo Transfection Reagent is a broad spectrum reagent that enables high efficiency oligonucleotide or oligoribonucleotide delivery in many cell types. TransIT-Oligo provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, ser…
TransIT-siQUEST® is a broad spectrum siRNA transfection reagent that enables high efficiency siRNA delivery and knockdown of target gene expression in many cell types including primary cells.
TransIT®-siPAK Trial Kit contains samples of both the TransIT®-siQUEST™ and TransIT-TKO® siRNA Transfection Reagents.Each unique formulation provides a distinct transfection profile for high efficiency, broadspectrum siRNA delivery into the cell type of interest.
Efficient siRNA or microRNA transfection. To attain efficient and reliable siRNA or microRNA transfection, we offer DharmaFECT transfection reagents. Thermo Scientific DharmaFECT 1 is our most all-purpose transfection reagent formulation; demonstrating efficient, low-toxicity delivery to over 35 cell types. DharmaFECT 1 achieves effective transfection of siRNA and microRNA reagents at low concentrations and with low cytotoxic…
SmartRNAplex miRNA Assays are your benchtop solution for miRNA profiling, using a familiar protocol, and simple acquisition software. SmartRNAplex microRNA Assays use proprietary Firefly™ technology, which exploits uniquely encoded hydrogel particles developed by innovators at Firefly BioWorks. The Firefly™ hydrogel particles are hydrophilic, bio-inert, flexible and porous, allowing target molecules to diffuse and bind in a…
High yield transient transfection and baculovirus titers in insect cells. Insect cell expression is a platform used to produce proteins with simple post-translational modifications. Transient transfection and recombinant baculovirus production are commonly used methods for insect cell expression.TransIT®-Insect Transfection Reagent is an animal-origin free transfection reagent specifically optimized for high gene expression i…
Research and Clinical Grade RetroNectin Reagent for Retroviral or Lentiviral Transduction. RetroNectin Reagent for retroviral or lentiviral transduction significantly increases transduction efficiency and results in enhanced gene transfer, especially in suspension and hematopoietic stem cells. RetroNectin Reagent is a chimeric peptide composed of E. coli-derived recombinant human fibronectin fragment that contains three funct…
Mouse ROSA26 safe harbor gene knock-in kit and clones. ROSA26 (also known as ROSAβgeo26 locus) in mice genome is first found in chromosome 6 in one particular strain of mice-named ROSAβgeo26-expressed β galactosidase from a randomly inserted transgene at high levels uniformly in nearly all tissues examined. This locus expresses one coding transcript and two noncoding transcripts, and only the non-coding transcripts are disrup…
GeneCopoeia offers complete custom services for CRISPR-Cas9-based targeted genomic modification, including CRISPR design and construction, donor design and construction, functional validations, stable cell line establishment, and transgenic mouse generation. Service Details: Validation Services Donor clone services Stable cell line services Transgenic mouse services Advantages: Fast delivery of CRISPR-Cas9 plasmids…
SMART (Switching Mechanism at 5’ End of RNA Template) is a unique technology that allows the efficient incorporation of known sequences at both ends of cDNA during first strand synthesis, without adaptor ligation. The presence of these known sequences is crucial for a number of downstream applications including amplification, RACE, and library construction. While a wide variety of technologies can be employed to take advantag…
With over 20 million pre-made clones representing nearly every known gene for most common organisms, Source BioScience are your perfect partner for quick and easy solutions for gene and protein expression research. Source BioScience customers can choose from individual pre-made clones or entire clone libraries from many of the most well-known and cited clone consortia and research groups for mammalian and non-mammalian specie…
