Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
High efficiency electroporation using any instrumentElectroporation using Ingenio Electroporation Kits and Solution affords increased gene expression in several different cell types with minimal toxicity. Ingenio Kits and Solution are compatible with multiple conventional electroporation instruments including Lonza Amaxa® Nucleofector®, Bio-Rad® Gene Pulser and Harvard-BTX® electroporators.Ingenio® Cuvettes Features:•High effi…
High efficiency electroporation using any instrumentElectroporation using Ingenio Electroporation Kits and Solution affords increased gene expression in several different cell types with minimal toxicity.Ingenio® Cell Droppers Features:•High efficiency electroporation of hard to transfect cell lines and primary cells•Compatible with most conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®•Idea…
High, reproducible protein yield in suspension CHO and 293 cellsTransIT-PRO® Transfection Kit consists of a DNA transfection reagent and boost combination specifically developed for mammalian protein production in suspension 293 and CHO derived cells.Reach critical stages faster. Decrease time to produce usable protein by maximizing target protein yields through transient transfection. The TransIT-PRO® Transfection Kit uses an…
A broad spectrum, low toxicity DNA transfection reagent optimized for 96-well plate applicationsTransIT®-Express Transfection Reagent is specifically developed for high-throughput transfection of plasmid DNA into mammalian cells. TransIT-Express Reagent provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use and repro…
A broad spectrum, low toxicity, DNA transfection reagentTransIT®-LT1 Transfection Reagent is a broad spectrum reagent that provides high efficiency plasmid DNA delivery in many mammalian cell types including primary cells. TransIT-LT1 is a low toxicity, serum-compatible transfection reagent that eliminates the need for any culture medium change.TransIT-LT1 is suitable for both transient and stable transfection and can be used…
A high performance, animal-free, broad spectrum DNA transfection reagentTransIT®-2020 Transfection Reagent is a high-performance, animal-origin free, broad spectrum reagent that provides exceptional transfection of plasmid DNA into mammalian cells. TransIT-2020 affords high levels of gene expression in typically hard-to-transfect cell types, including primary cells and is suitable for both transient and stable transfection.Tra…
A high efficiency DNA transfection reagent optimized for Jurkat and other hard-to-transfect cell typesTransIT®-Jurkat Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in Jurkat cells and cell types of associated lineage.TransIT-Jurkat reagent also works well for additional cell lines of hematopoietic origin such as THP-1(human acute monocytic leukemia cell line) and R…
A high efficiency, low toxicity, DNA transfection reagent optimized for prostate cell typesTransIT®-Prostate Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in Prostate cell types. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use and reproducibil…
A high efficiency, low toxicity, DNA transfection reagent optimized for various neural cell typesTransIT-Neural® Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in various neural cell types. TransIT-Neural Reagent provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simpli…
A high efficiency, low toxicity, DNA transfection reagent optimized for CHO cell typesTransIT®-CHO Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in CHO cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use…
A high efficiency, low toxicity, DNA transfection reagent optimized for 3T3 cell typesTransIT®-3T3 Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in NIH 3T3 cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of…
A high efficiency, low toxicity, DNA transfection reagent optimized for COS cell typesTransIT®-COS Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in COS cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use…
A high efficiency, low toxicity, DNA transfection reagent optimized for keratinocyte cellsTransIT®-Keratinocyte Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in keratinocyte cell types such as NIKS (Near-Diploid Immortalized Keratinocytes) and primary keratinocytes. TransIT-Keratinocyte Reagent provides all the attributes of the trusted TransIT series of transfecti…
A high efficiency, low toxicity, DNA transfection reagent optimized for HeLa cellsTransIT-HeLaMONSTER® Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in HeLa cells and cell types of associated lineage. This kit provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity o…
Superior DNA transfection of breast cancer cell typesTransIT-BrCa Transfection Reagent is the first dedicated breast cancer cell line specific reagent available to researchers today. While it delivers efficiently to many breast cancer cell types, it also maintains low cellular toxicity resulting in reduced experimental biases. TransIT®-BrCa Transfection Reagent is specifically optimized to provide exceptional transfection effi…
A nontoxic, "Quick Recovery" In Vivo delivery solution that delivers DNA or siRNA to the livers of laboratory mice using methods including hydrodynamic tail vein injection.The TransIT-QR (Quick Recovery) Hydrodynamic Delivery Solution is designed specifically for the safe and efficient delivery of nucleic acids into laboratory mice using the hydrodynamic tail vein injection procedure. This formulation is optimized for efficien…
An "Enhanced Expression" In Vivo delivery solution that delivers DNA to the livers of laboratory mice using methods including hydrodynamic tail vein injection.The TransIT-EE (Enhanced Expression) Hydrodynamic Delivery Starter Kit is designed specifically for the delivery of DNA expression vectors into live laboratory mice using the hydrodynamic tail vein injection procedure. This formulation is designed to produce high-level g…
A high efficiency, low toxicity, large RNA transfection reagent for mammalian cells.TransIT®-mRNA Transfection Kit is designed to transfect RNA into a broad range of cell types with minimal cellular toxicity. RNA delivery avoids transcriptional regulation effects by directly delivering the RNA to the cytoplasm for expression. The TransIT-mRNA Transfection Kit can be used to deliver a variety of RNA molecules including mRNAs an…
A high efficiency, low toxicity, transfection reagent optimized for oligonucleotide delivery into a wide range of cell typesTransIT®-Oligo Transfection Reagent is a broad spectrum reagent that enables high efficiency oligonucleotide or oligoribonucleotide delivery in many cell types. TransIT-Oligo provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, ser…
TransIT-siQUEST® is a broad spectrum siRNA transfection reagent that enables high efficiency siRNA delivery and knockdown of target gene expression in many cell types including primary cells.
TransIT®-siPAK Trial Kit contains samples of both the TransIT®-siQUEST™ and TransIT-TKO® siRNA Transfection Reagents.Each unique formulation provides a distinct transfection profile for high efficiency, broadspectrum siRNA delivery into the cell type of interest.
Efficient siRNA or microRNA transfection. To attain efficient and reliable siRNA or microRNA transfection, we offer DharmaFECT transfection reagents. Thermo Scientific DharmaFECT 1 is our most all-purpose transfection reagent formulation; demonstrating efficient, low-toxicity delivery to over 35 cell types. DharmaFECT 1 achieves effective transfection of siRNA and microRNA reagents at low concentrations and with low cytotoxic…
SmartRNAplex miRNA Assays are your benchtop solution for miRNA profiling, using a familiar protocol, and simple acquisition software. SmartRNAplex microRNA Assays use proprietary Firefly™ technology, which exploits uniquely encoded hydrogel particles developed by innovators at Firefly BioWorks. The Firefly™ hydrogel particles are hydrophilic, bio-inert, flexible and porous, allowing target molecules to diffuse and bind in a…
The TnT® Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression. These cell-free expression systems combine the RNA Polymerase, nucleotides, salts, amino acids and Recombinant RNasin® Ribonuclease Inhibitor with the reticulocyte lysate solution to form a single TnT® Quick Master Mix. The TnT® Quick Coupled Transcription/Translation System is ava…
The Kazusa ORFeome Project was initiated to create a collection of human and mouse ORFs to enable the larger research community with a readily accessible, highly validated ORFeome. Kazusa DNA Research Institute (KDRI) is an academic Japanese research institute dedicated to genome-scale studies and the function of individual genes. KDRI is an active member of the global ORFeome Collaboration, and are experts in HUGE (Human Uni…
