Enzymes Products & Reviews

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Enzymes are macromolecular biological catalysts used across life science, pharmaceutical and clinical research. Various enzymes can be purchased for research applications including ligases, high fidelity polymerases, nuclease, protease, restriction enzymes, tissue dissociation enzymes and enzyme inhibitors. Find the best enzymes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Heat&Run gDNA Removal Kit

ArcticZymes

The Heat&Run® gDNA removal kit removes contaminating gDNA from RNA prior to RT-qPCR. The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at low temperatures (5 minutes at 58ºC or 15 minutes at 55ºC). Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.

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Endonucleases DNA-specific

ArcticZymes

Double-Strand Specific dsDNase from ArcticZymes is ideal for removing contaminating DNA from PCR master mixes, preventing false positives in bacterial typing. Recombinantly produced in Pichia pastoris, it cleaves dsDNA with 5000-fold higher specificity than ssDNA. Easily inactivated by moderate heat (65°C), it allows for direct addition of DNA templates post-cleanup, ensuring accurate PCR results.

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Recombinant Active Enzymes

Sino Biological

SignalChem Biotech (part of Sino Biological) offers a broad range of recombinant protein and lipid Kinases of superior purity and activity to assist the global academic research and drug discovery efforts with the focus on Kinases.

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AZtaq™ DNA Polymerase

ArcticZymes

AZtaq™ DNA Polymerase, derived from Thermus aquaticus, is a high-quality, thermostable enzyme ideal for PCR applications. It catalyzes the synthesis of complementary DNA using a primed template and has 5’-3’ exonuclease activity but lacks 3’-5’ proofreading. AZtaq is compatible with dUTP, allowing efficient carry-over contamination removal when used with Cod UNG.

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Endonucleases Non-Specific, HL-SAN

ArcticZymes

HL-SAN efficiently removes nucleic acids from buffers in protein purification, excelling in high-salt conditions to reduce aggregation. Ideal for removing nucleic acids from proteins with high DNA/RNA affinity, it performs well during lysis, early purification stages, and high-salt eluates. This cold-adapted enzyme works effectively at ambient temperatures and during overnight digestion at 4°C. Triton free available.

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Bioprocessing Salt Active Nucleases - Physiological Conditions

ArcticZymes

Nonspecific endonuclease is highly active at physiological conditions (125–200 mM) for DNA removal during protein production, vaccine manufacturing, and viral vector preparation. M-SAN High Quality can be used directly in cell media, which improves efficiency and yield. Designed to replace Benzonase without workflow changes. M-SAN HQ excels in ensuring higher purity and efficiency. High purity (≥ 99%). Compatible with ELISA ki…

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Endonucleases DNA-specific, HL-dsDNase

ArcticZymes

HL-dsDNase efficiently removes genomic DNA from RNA with minimal impact on RNA quality, reducing 50 ng of gDNA to undetectable levels by qPCR. Easily inactivated by 5-minute incubation at 58°C, it’s ideal for RNA prep, minimizing degradation. It's also effective for removing external DNA from samples before metagenomic sequencing and STR-profiling, for fast, efficient alternative to traditional methods. Triton free available.

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ApopTag® TdT Enzyme

Merck

ApopTag® Terminal deoxynucleotidyl Transferase (TdT) is a recombinant enzyme intended for use in labeling the 3'-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag® In Situ Apoptosis Detection Kits

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Antipain

Merck

Antipain is a serine and cysteine reversible protease inhibitor. It inhibits papain and trypsin. Plasmin is inhibited to a small extent as well. More specific than leupeptin for papain and trypsin. Source: Microbial origin. Not synthetically made.

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KOD HiFi DNA Polymerase

Merck

Pure recombinant high-fidelity DNA polymerase from Thermococcus kodakaraensis KOD1KOD HiFi DNA Polymerase* is a recombinant form of Thermococcus kodakaraensis KOD1 DNA polymerase. KOD HiFi is a high fidelity thermostable polymerase that amplifies target DNA up to 6 kbp in length with superior accuracy and yield. The enzyme’s 3′→5′ exonuclease-dependent proofreading activity results in a lower PCR mutation frequency than any ot…

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KOD XL DNA Polymerase

Merck

High-performance enzyme blend for long and accurate PCRKOD XL DNA Polymerase* is an optimized blend of KOD HiFi DNA Polymerase and a mutant form of KOD HiFi that is deficient in 3′→5′ exonuclease activity. This enzyme mixture is designed for reliable amplification of long, complex targets with robust yield and high accuracy. It can also be used for incorporation of dITP, dUTP and derivatized dNTPs in PCR amplicons. KOD XL DNA…

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AmpliTaq® DNA Polymerase

Thermo Fisher Scientific

AmpliTaq® DNA Polymerase is a 94 kDa ultra-pure, gelatin-free, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus Taq DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the polymerase chain reaction (PCR) process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lo…

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RNA Polymerase II Toolbox

Chromotek GmbH

RNA polymerase II features a large carboxyl-terminal repeat domain (CTD) that consists of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Differential phosphorylation in this CTD orchestrates accessibility for various co-factors of the transcription complex. Using the phosphorylation specific antibodies it is now possible to determine specific phosphorylation states. In addition to phosphorylation specific antib…

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