Life Sciences Products & Reviews

BRET-Based Assays to Detect Protein:Protein Interactions in Live Cells

The small size and bright luminescence of NanoLuc® luciferase bring exquisite sensitivity to many applications. From NanoBRET™ technology, a BRET-based method to study protein:protein and protein:small molecule interactions, to NanoLuc® Binary Technology (NanoBiT), NanoLuc is the cornerstone for studying genetic responses and protein dynamics in cells at physiologically relevant levels. The flexibility of NanoLuc® technologies…

High-Throughput Extraction of DNA from FFPE Samples Robust, precipitation-free protocol, no chance of lost pellets High yields of pure DNA from FFPE samples without using xylene or other hazardous chemicals Ideal for use in downstream applications including qPCR and next-generation sequencing (NGS)

The Wizard MagneSil Tfx™ System provides a simple, rapid method for isolating transfection-quality plasmid DNA in a 96-well, high-throughput format. The use of paramagnetic particles for lysate clearing as well as DNA capture circumvents the need for centrifugation or vacuum manifolds, allowing DNA purification to be completely automated.

The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells. Total RNA can be purified from 96 samples in less than an hour without centrifugation. The system incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol:…

The Wizard ® SV 96 Genomic DNA Purification System provides a high-throughput, membrane-based technique for consistent preparation of genomic DNA from cultured cells and tissue, including mouse tails. Amplifiable genomic DNA can be isolated from up to 5 × 106 cells, 20mg of tissue or up to 1.2cm of a mouse tail tip without a centrifugation clearing step.

Column-based method for rapid concentration and cleanup of DNA.

Column-based method for rapid concentration and cleanup of RNA.

Isolate High-Molecular-Weight DNA for Long-Read-Sequencing applications.

LDH-Glo™ Cytotoxicity Assay detects cell death from a small number of cells, including 3D cell cultures, based on the release of lactate dehydrogenase.

The RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay provides a no-wash, one-step Annexin V assay that allows continual monitoring of cell state to determine apoptotic onset using a plate-reading luminometer.

The GSH/GSSG-Glo™ Assay is a bioluminescent assay to detect and quantitate total glutathione ratios.

The Caspase-Glo ® 1 Inflammasome Assay  directly measures inflammasome activation with a simple add-mix-measure protocol.

Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism of action of antibodies through which virus-infected or other diseased cells are targeted for destruntion by components of the cell-mediated.

The TNT® Coupled Reticulocyte Lysate Systems offer researchers an alternative for eukaryotic cell-free protein expression: a single-tube, coupled transcription/translation system. The TNT® Lysate Systems greatly simplify the process and reduce the time required to obtain in vitro translation results.

Wizard® Plus Megapreps DNA Purification System provides a simple and rapid method for large-scale purifications of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. The system yields greater than one milligram of high-copy-number plasmid DNA (200–20,000bp) from a 1,000ml culture in less than th…

ProteaseMax™ Surfactant is a high-quality reagent for use in protein and peptide sample preparation.   ProteaseMax™ Surfactant solubilizes proteins and ensures fast and improved protein digestion with trypsin or chymotrypsin. It is designed to degrade over the course of a digestion reaction, yielding products that are compatible with downstream methods such as mass spectrometry (MS) and liquid chromatography (LC). For…

HaloLink - Promega Magnetic Beads for Covalent immobilization of HaloTag® fusion proteins.   Features of the Promega HaloLink Magnetic Beads: • Magnetic-Based: Easy and quick immobilization of HaloTag® fusion proteins with minimal loss of sample material. • Covalent Bond: Allows stringent washing of immobilized HaloTag® fusion protein, removing nonspecific proteins and enhancing overall data.  • Multiple Appli…

The EUROLabWorkstation ELISA provides fully automated processing of ELISA in laboratories with high sample throughput. The system has a high capacity of up to 15 microplates and can process more than 200 tests per hour. Ten washable needles, complementary accessories and a separate robotic arm for handling the microplates enable unparalleled sample throughput. Intuitive software guides the user through all work steps.

Developing a precise, accurate, and reliable assay is never easy. From raw material sourcing, to technical considerations, to optimization and ultimate scale-up, a development team counters challenges that strain resources and delay timetables. Savvy developers recognize that assay development and optimization move smoother when optimal raw materials and expert support are identified early in the process.

Predesigned probe assays for gene expression analysis

Custom adapters for multiplex next-generation sequencing

Predesigned primers for gene expression analysis

Specialized purification to ensure high performance

Larger quantities for in vivo, high throughput and commercial projects