Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
Thermo Scientific rDNase I is produced through a process that contains no animal by-products. The enzyme is provided free from any detectable RNase activity or animal derivatives. rDNase I has a molecular weight of approximately 30kDa. As an endodeoxyribonuclease, DNase I hydrolyses phosphodiester linkages yielding di-, tri-, and oligonucleotides with a 5' phosphate and a free 3' hydroxyl. DNase I has been shown to act on sing…
Thermo Scientific IPTG (isopropyl-ß-D-thiogalactopyranoside) is a chemical analogue of galactose, which cannot be cleaved by ß-galactosidase. It acts as an inducer of the lac operon.
Full-, semi- or non-skirted 96-well PCR plates with different profiles. Ultra-rigid plates for robotic applications.
Thermo Scientific Anchored Oligo-dT primers are specifically designed for synthesising long cDNAs from mRNAs carrying poly-A tails. Since this primer only anneals to the poly-A tail junction of the mRNA template, it eliminates transcription through the poly-A tail, providing more effective cDNA synthesis.
dNTP mixes – supplied in a premixed aqueous solution of dATP, dTTP, dCTP, and dGTP. Thermo Scientific produces extremly pure dNTPs, and the company is one of only a few primary manufacturers in the industry. Thermo Scientific dNTPs are supplied in aqueous solutions titrated to pH 7.0 with NaOH. dNTPs are available individually as nucleotide mixes at convenient concentrations for direct use in PCR, reverse transcription, label…
PCR Cloning Kit-Seamless DNA Cloning with In-Fusion HD Cloning Plus
Assess serum Gc-globulin levels to obtain important information about prognosis and progress in critically ill patients suffering from fulminant liver failure, Acetaminophen overdose, multiple trauma and Sepsis. Using our Gc-globulin (Actin-free) ELISA Kit you can determine Gc-globulin levels in serum or plasma in less than 1 hour. The fast procedure and easy-to-use ELISA format makes this kit ideal for prognostic assessment a…
Custom DNA oligos—manufactured and shipped the same day you order
The Power SYBR® Green RNA-to-CT™ 1-Step Kit provides high sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction kit. A novel formulation helps reduce false-positive results caused by primer-dimers that are common in SYBR® Green-based assays. This significantly improves data accuracy and enables the reliable detection of low expressing targets or targets present at low amounts. Minimized hands-on time wit…
The SYBR Green RT-PCR Kit provides all the necessary components to perform both one- and two-step RT-PCR, which makes it ideal for evaluation between the two RT-PCR protocols.
The Thermo Scientific Total RNA Isolation Reagent (TRIR) is designed for the extraction of total RNA from a variety of sources. A biological sample is homogenised with the reagent using a glass-Teflon® homogeniser. After extraction with chloroform, the homogenate separates into two phases. RNA remains in the aqueous phase while DNA and proteins are extracted into the organic phase. The RNA is precipitated from the aqueous phas…
The High Capacity RNA-to-cDNA Master Mix optimizes cDNA synthesis for high-throughput, real-time gene expression analysis. • Single tube, complete first-strand cDNA synthesis master mix reduces experimental variability and streamlines workflow • 5X concentration provides robust results even with dilute RNA samples and rare or abundant transcripts • Liquid format at –10 and –20°C eliminates freeze/thaw cycles and maximizes chem…
The Veriti™ 384-Well Thermal Cycler (0.02 mL) delivers the proven reliability of Applied Biosystems 9700 GeneAmp® instruments with the throughput you need for large experiments. • Network up to 12 instruments together using standard Ethernet cables and a router (not provided) • From a single, networked Veriti™ instrument, start a run on up to 12 Veritis • Conveniently place instruments side-by-side due to front to back venti…
The Veriti™ 60-Well Thermal Cycler delivers the proven reliability of Applied Biosystems 9600 and 9700 GeneAmp® instruments with the 0.5 mL format you need to accomodate larger reaction volumes. . Additionally, the powerful user interface through the color touch screen simplifies the setup and use of the Veriti 60-Well Thermal Cycler. Optional setups for fast or standard PCR methods provide you the flexibility to shorten your…
Whether your profiling experiment requires ultimate sensitivity, broad coverage or both, Megaplex™ Primer Pools provide the flexibility to reach your research goals. Megaplex Primer Pools deliver comprehensive coverage of Sanger miRBase v10, and when used with TaqMan® MicroRNA Arrays, offer an ideal miRNA profiling solution. Optional Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed miRNAs en…
A novel thermoreversible gel mountant for extended immobilization and imaging.
The 1 kb DNA Ladder contains 13 discrete DNA fragments ranging in size from 300 bp to 10,000 bp. This marker is ideal for the size determination of digested DNA. Two Ready-to-Use DNA Size Markers for Gel Electrophoresis. The 100 bp Ladder and 1 Kb Ladder represent the two most popular markers used for DNA fragment size determination. These markers are formulated to run accurately and to provide crips band patterns. The two m…
The 100 bp DNA Ladder contains 12 discrete DNA fragments ranging in size from 100 bp to 3,000 bp. This marker is ideal for the size determination of PCR products. Two Ready-to-Use DNA Size Markers for Gel Electrophoresis. The 100 bp Ladder and 1 Kb Ladder represent the two most popular markers used for DNA fragment size determination. These markers are formulated to run accurately and to provide crisp band patterns. The two…
For purification of up to 20 µg molecular biology grade plasmid DNA
AxyPrep Bacterial Genomic DNA Miniprep Kit - Suitable for the rapid isolation of up to 20 mg of genomic DNA from 1.0×109 bacterial cells. Following lysis, separation of the genomic DNA from proteins, polysaccharides and lipids with the AxyPrep Plasmid MiniPrep Kit is achieved by a unique phase-partition step. The genomic DNA in the lower phase is selectively bound to a special Axygen AxyPrep column for further purification a…
Format of the AxyPrep™ Multisource Total RNA Maxiprep Kit: • Maxi column AxyPrep™ Multisource Total RNA Maxiprep Kit Starting Materials: • Animal tissues • Plant tissue • Cultured cells • Bacteria • Yeast • Filamentous fungi Features of the AxyPrep™ Multisource Total RNA Maxiprep Kit: • Rapid spin and vacuum protocols • No alcohol precipitation • No column clogging • Dramatic reduction in DNA carryover • Higher yields, higher…
