Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
The QuickPick Plant DNA kit is intended for the purification of plant DNA. Designed for use with the PickPen magnetic tool, the kit uses MagaZorb™ technology to purify DNA from sample amounts of up to 25 mg (up to 15 mg when working with 96-well microplates) of plant material.
The QuickPick Plant DNA kit is intended for the purification of plant DNA. Designed for use with the PickPen magnetic tool, the kit uses MagaZorb™ technology to purify DNA from sample amounts of up to 25 mg (up to 15 mg when working with 96-well microplates) of plant material.
This ready-to-use kit is intended for the purification of poly (A)+ mRNA from cultured cells, animal and plant tissue and total RNA, and is used together with the PickPen magnetic tool. Each purification takes only 10 minutes. The mini kit is designed to be used with starting material of up to 1 million cells, 30 mg animal tissue, 50 mg plant tissue or 50 µg total RNA per prep. The mini kit contains reagents and tips for 24 pr…
This ready-to-use kit is intended for the purification of poly (A)+ mRNA from cultured cells, animal and plant tissues, and is used together with the PickPen magnetic tool. Each purification takes only 10 minutes. The mini kit is designed to be used with starting material of up to 100.000 cells, 3 mg animal tissue or 5 mg plant tissue per prep. The micro kit contains reagents and tips for 48 preps.
This ready-to-use kit is intended for the purification of poly (A)+ mRNA from cultured cells, animal and plant tissues, and is used together with the PickPen magnetic tool. Each purification takes only 10 minutes. The mini kit is designed to be used with starting material of up to 1000 cells, 30 µg animal tissue or 50 µg plant tissue per prep. The mini kit contains reagents and tips for 96 preps.
For use with PickPen magnetic tools when not working with QuickPick products, PickPen standard tips withstand autoclaving to ensure DNAase/RNAase free conditions. To avoid sample cross-contamination, new tips should be taken for each sample. Note that Bio-Nobile's QuickPick kits include all tips needed for the number of preparations specified for the kit.
For use with PickPen magnetic tools when not working with QuickPick products, DNAase/RNAase free PickPen tips are supplied in a convenient plastic pack. Note that Bio-Nobile's QuickPick kits include all tips needed for the number of preparations specified for the kit.
For convenient handling and storage of PickPen tips, the PickPen tip box will hold 96 tips in 8 x 12 configuration in a vertical position allowing them to be picked up easily with PickPen magnetic tools.
DATAN 3.0OverviewWith DATAN 3.0 characterization of chemical equilibria and chemical reactions becomes easy. DATAN exploits the great advantage of collecting entire spectra and even higher order experimental data when studying chemical samples. Behind a user friendly interface state of the art algorithms, several of which are proprietary to MultiD, are used to extract chemical information about the samples with minimum assumpt…
ViBE Protein Analysis PlatformPowered by AMMP Technology, the ViBE platform allows rapid design and configuration of protein assays to achieve highly-sensitive detection and quantitation of proteins in complex biological samples with real-time results. The ViBE platform … • Open assay design allows use of your own antibodies and the ability to define sensitivity, dynamic range, and speed requirements for efficient process…
CHEMICON's ES Cell Characterization Kit (Catalog number SCR001) is a specific and sensitive tool for the phenotypic assessment of the differentiation status of ES cells by measuring their AP activity, cell-surface stage-specific antigens (SSEA-1, SSEA-4) as well as expression of TRA-1-60, TRA-1-81 antigens.
The ES Cell Marker Sample Kit includes six monoclonal antibodies for the analysis of ES cell marker expression. CHEMICON®'s ES Cell Marker Sample Kit (Catalog number SCR002) contains monoclonal antibodies for the detection of the cell-surface Stage-Specific Embryonic Antigens (SSEA-1, SSEA-3 & SSEA-4), expression of TRA-1-60, TRA-1-81 antigens and Oct-4. This kit permits the phenotypic analysis of the differentiation status of…
EMD Millipore's Alkaline Phosphatase Detection Kit is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.
EMD Millipore's Mesenchymal Stem Cell Characterization Kit (Catalog Number SCR018) contains a panel of positive and negative selection markers for the characterization of the mesenchymal stem cell population in rat. Positive cell markers include antibodies directed against cell-surface molecules (integrin beta1 and CD54) (Pittenger, M. F., and Marshak, D. R., & Pittenger, M.F., et al.) present on mesenchymal stem cells along w…
CHEMICON®'s Mesenchymal Stem Cell Osteogenesis Kit contains all the reagents necessary to readily differentiate mesenchymal stem cells to an osteogenic lineage as assessed by Alizarin Red staining. Reagents in the kit include two ECM coating molecules (collagen Type I and vitronectin) that have been shown to promote osteogenic differentiation of mesenchymal stem cells (Salasznyk, 2004) along with inducing reagents, dexamethaso…
The Rodent Neuron Differentiation Kit is a system designed for the optimal differentiation of neural stem cells derived from rodents into neurons. Included in the kit are two neural inducers that when added to the Neural Stem Cell Basal Medium allows for the preferential differentiation of rodent neural stem cells to a neuronal lineage. Other components of the kit include reagents for the fixation and two antibodies for the im…
The Pancreatic Islet Cell Characterization Kit (SCR045) provides a convenient set of validated antibodies that allows researchers to reliably identify mature pancreatic islets cells. Along with antibodies generated against discrete hormones secreted by alpha, beta, delta and gamma cells of the pancreatic islets, the kit includes PDX-1 (pancreatic duodenal homeobox gene-1), a master regulator of islet cell development and GLUT-…
Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPflash which contains wild type TCF binding sites.
The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. Cells are cultured in microplates, treated with agents that induce DNA damage or apoptosis, which stimulates H2A.X phosphorylation. Cells are then fixed and permeabilized in preparation for staining and detection. Histone H2A.X phosphorylated at serine 139 is detected by…
TOPglow (TCF reporter plasmid): Transfection grade T cell factor (TCF) reporter plasmid containing four copies of the Tcf binding site (wt) upstream of E1B TATA box and Luciferase open reading frame.
The ApopTag ISOL Fluorescence Apoptosis Detection (DNase Types I & II) technique is based upon the biochemical specificity of the enzymes T4 DNA ligase and Vaccinia Virus DNA Topoisomerase I and the unique, dual labeled, dual hairpin oligonucleotide
The CHEMICON APO-DIRECT™ Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains all the reagents required for measuring apoptosis in cells including positive and negative control cells; washing, reaction, and rinsing buffers; and propidium iodide/RNase A solution for counter staining the total DNA.
The MitoLight™ Apoptosis Detection Kit utilizes a lipophilic cation, termed as MitoLight™, as a mitochondrial activity marker. MitoLight™ is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. The monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential. MitoLight™ partitions differently i…
The CHEMICON In Situ FLICA Pan-Caspase Detection Kit is a fluorescent-based assay for detection of active caspases in cells undergoing apoptosis. The kit is for research use only. Not for use in diagnostic or therapeutic procedures.
