Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
The VITEK 2 is a fully automated system that performs bacterial identification and antibiotic susceptibility testing. The system also includes a highly advanced software system that then interprets the identified bacteria's antibiotic resistance patterns.
High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System(a,b,c) is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample…
The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe PCR System will faithfully amplify your template every time. The system includes the FailSafe™ PCR Enzyme Mix and a meticulously tested set of 12 FailSafe™ PCR “PreMixes” that cover a matrix of enzyme-specific PCR conditions that are optimal for amplifying different se…
Versatile — works with a broad range of cell lines Reproducible — produces consistent results, lot-to-lot, plate-to-plate, and well-to-well Efficient — performs with high siRNA transfection efficiency, allowing use of low siRNA concentrations to minimize nonspecific effects Fast — reverse-transfects cells as they are plated, saving a day
For the characterization of cell death through agarose-gel electrophoresis.PrincipleBlood or cell lysis is accomplished by incubating the sample with the special Binding/Lysis Buffer. The sample is centrifuged through a column that contains glass fiber fleece. In the Binding/Lysis Buffer, nucleic acids quickly and preferentially bind to the surface of the glass fibers. The Washing Buffer rinses away salts, proteins, and other…
Application The Cytotoxicity Detection KitPLUS (LDH) is a fast, sensitive, and simple method to quantitate cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells using the 96-well or 384-well plate format. The kit can be used in many different in vitro cell systems when damage to the plasma membrane occurs. For example: Detection and quantification of cell-mediated cytotoxicity. Deter…
Roche FuGENE 6 Transfection Reagent is a non-liposomal multicomponent reagent well-suited for experiments involving cellular analysis. Due to the simple methodology of FuGENE 6 Transfection Reagent, minimal optimization, low cytotoxicity, and ability to provide high transfection efficiency even in the presence of serum, it is one of the standard transfection reagents in many different laboratories around the world. With a ment…
FastStart Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. This enzyme delivers superior results thanks to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is inactive at temperatures below 75°C, but is activated by a 2- to 4-minute incubation step at 95°C. PCR Hot Start PCR RT-PCR Benefits Insist on higher specificity, sensitivity…
Upgrade from the Expand Long Template PCR System and choose the new Expand Long Range dNTPack for consistent amplification of long PCR products. Generate PCR products of 5 to 25 kb from genomic DNA with this next-generation system from the company that pioneered long-template PCR. This specially designed PCR system combines a single buffer set for all product sizes, ultrapure PCR-grade nucleotide mix, and an optimized blend of…
The kit is designed for the preparation of total RNA mini-preps (free of contaminating DNA) from cultured cells. Other sample materials like blood, yeast, and bacteria require a pre-lysis treatment. The purified and intact RNA is of high quality, and is suitable for RT-PCR and northern blotting, RNase protection, or primer-extension experiments. Up to 24 samples can be simultaneously processed in approximately 1 hour. Benefit…
The new Finnigan LCQ Advantage MAX delivers high productivity LC/MS/MS analysis in a routine HPLC environment. Product detail: The Finnigan LCQ Advantage is the workhorse ion trap mass spectrometer for high productivity LC/MS/MS results and is easily upgraded to MSn performance. The Finnigan LCQ Advantage MAX now features the universal Ion Max source that allows simple tool-less switching of ionization probes. Ion Sweep Gas…
For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.
The Advantage® RT-for-PCR Kit allows first-strand cDNA synthesis from either total or poly A+ RNA. With this kit, you can obtain sufficient quantities of cDNA for PCR from nanogram quantities of any RNA. The Advantage® RT-for- PCR Kit offers several advantages: Longer oligo(dT) primers provide more effective cDNA priming. Recombinant-derived RNase inhibitor minimizes RNA degradation. Each kit contains everything you ne…
The HRP is purified by repeated affinity chromatography of root extracts from horseradish. The result is an enzyme of high specific activity. Our horseradish peroxidase is purified in large scale by the use of benzhydroxamic acid agarose giving a product of high purity and specific activity. The product consists mainly of the basic isoenzymes and are supplied as lyophilized powder. Activity: >250 U/mg (25°C, Guaiacol as hydr…
Piko™ Thermal Cyclers - high thermal performance in a tiny footprint. Piko, inspired by the Italian word “piccolo,” meaning tiny, aptly describes Finnzymes’ new PCR system. At the heart of the Piko Thermal Cycler lies our unique heat pump. Designed to deliver bridled power, it results in industry-leading thermal uniformity, accuracy, ramp rates and settling times. This not only allows for PCR protocols in as little as 10 minut…
PerkinElmer LAMBDA 1050+ UV/Vis/NIR spectrophotometers are designed to offer the highest performance and flexibility to analyze a wide range of sample types, including analysis of coatings, high performance glass, solar, and advanced materials and components in both research and manufacturing. The LAMBDA 1050+ meets and often exceeds industry standards for ultra-high performance, flexibility, and convenience. The latest gen…
The Sirocco™ plates are the most technologically advanced protein precipitation plates in the market and enable high-throughput “in-well” protein precipitation of biological samples without the fear of cloudy filtrates or clogged devices. It also provides optimum performance in faster processing time by reducing steps in ‘in-well’ sample processing and complete recovery of clean filtrate from smaller plasma sample volumes.
Alpha is a bead-based, no-wash luminescent technology. When Alpha Donor and Acceptor beads are brought together through binding to target, a cascade of chemical reactions is set in motion, creating an amplified signal. These versatile beads are conjugated with various biomolecules that enable detection of unique biological events, offering maximum flexibility.
Applied Biosystems’ latest entry-level real-time PCR instrument, the StepOne™ Real-Time PCR System, makes it simple and easy to get high-quality real-time PCR results. This remarkably simple real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers new to real-time PCR.
The Oasis® uElution plate format combined with the Oasis chemistries allow elution volumes as low as 25uL, eliminating the time consuming evaporation step, producing very clean extracts with a 5 to 10 fold increase in concentration.The Oasis® family of sample extraction products is designed to simplify and improve your sample preparation by combining the right sorbent chemistry, device format and methodology. Achieve robust, s…
The AXIMA range of MALDI-TOF-MS systems delivers uncompromised data quality for biochemical, chemical and life science researchers.
The Thermo Scientific LTQ Orbitrap XL ETD is the most powerful instrument for proteomics analyses: It combines three different and complementary fragmentation techniques CID, HCD, and ETD with the proven benefits of Orbitrap technology. It is the most comprehensive solution for complex PTM analysis, intelligent sequencing of peptides, top-down and middle-down analysis, and protein quantitation via stable isotope labelling su…
Thermo Scientific LTQ Orbitrap XL - The Most Confident Protein Identification The Thermo Scientific LTQ Orbitrap XL hybrid FTMS (Fourier Transform Mass Spectrometer) supports a wide range of applications from routine compound identification to the most challenging analysis of low level components in complex mixtures. The LTQ Orbitrap XL is based on the fast and highly sensitive Thermo Scientific LTQ XL linear ion trap and th…
The CellTiter-Blue® Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin). Nonviable cells rapidly lose metabolic capacity and thus do not generate a fluorescent signal. The homogeneous assay procedure involves adding the single reagent directly to cells cultur…
The PureYield™ Plasmid Midiprep System is designed to isolate transfection-quality plasmid DNA. The system provides a rapid method for purification of 100–200μg of plasmid DNA from 50ml bacteria culture. Plasmid DNA can be purified in as little as 30 minutes with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows…
