The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours.
Zeba Spin Desalting products are polypropylene devices containing a proprietary high-performance size-exclusion chromatography resin that provides an excellent protein desalting and recovery in a centrifuge format.
The BenchMark Pre-Stained Protein Ladder is a standard used to monitor electrophoresis or electrophoretic transfer from gel to membrane in Western blots.
Spectra Multicolor Broad Range Protein Ladder is a 4-color protein standard containing 10 prestained proteins (10 to 260kDa) for use in gel electrophoresis and Western blotting.
The Mark12 Unstained Standard allows the closest estimation of molecular weight of your sample protein over a wide molecular weight range.
The NativeMark Unstained Protein Standard is a ready-to-use protein standard designed for molecular weight estimation of proteins using native gel electrophoresis.
The Pierce ESI Negative Ion Calibration Solution is a mixture of highly purified ionizable molecules designed for negative mode calibration of Thermo Scientific mass spectrometer instruments.
Lys-C Protease, MS Grade is a highly purified, mass-spectrometry-grade, native endoproteinase validated for maximum activity and stability in proteomic applications.
SuperSignal ELISA Femto Substrate is formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.
RIPA Lysis and Extraction Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.
T-PER Tissue Protein Extraction Reagent enables mild extraction of total protein from tissue samples.
Mem-PER Plus Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of integral and membrane-associated proteins using a simple reagent-based procedure.
Spectra Multicolor High Range Protein Ladder is a mixture of eight (8) blue-, green-and orange-stained proteins (40 to 300kDa) for use as size standards for high-MW proteins in gel electrophoresis and Western blotting.
The Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins with the exclusion of intracellular proteins.
Pierce Coomassie Plus Protein Assay is a ready-to-use, reducing agent-compatible, improved Bradford assay reagent to quickly measure (A595nm) total protein concentration compared to a protein standard.
Pierce Abundant Protein Depletion Spin Columns are optimized to decrease the abundant albumin and antibody components of human plasma samples in preparation for mass spectrometry or protein gel electrophoresis.
Add mass spectrometry (MS) to your IC and LC analyses for access to the valuable data that only MS can provide. The new, easy-to-use Thermo Scientific™ ISQ™ EC Single Quadrupole Mass Spectrometer seamlessly integrates with ion chromatography (IC) and high performance liquid chromatography (HPLC) systems. Both novices and experts alike can quickly master MS to gain more insights from every sample.
JC-1 is a membrane permeable dye widely used for determining mitochondrial membrane potential in flow cytometry and fluorescent microscopy. This dye can selectively enter the mitochondria where it reversibly changes coloras membrane potentials increase (over values of about 80-100 mV). When excited at 488 nm, the emission shift goes from 530 nm (monomeric JC-1) to 590 nm (aggregate JC-1), which are easily detected using filter…
eFluor 514 Calcium Sensor Dye is a membrane-permeable dye used for monitoring changes in intracellular free calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. When it enters a cell, cellular esterases cleave the dye, resulting in a membrane-impermeable dye that fluoresces at ~520 nm. eFluor™ 514 Calcium Sensor Dyeis a commonly used dy…
DRAQ5, an anthraquinone dye with high affinity for double-stranded DNA, is a membrane-permeable dye that can label live or fixed/dead cells. The dye is used to distinguish nucleated and non-nucleated cells in flow cytometry, to report nuclear DNA content for ploidy and cell cycle analysis and as a nuclear counterstain nuclei in fluorescent microscopy.DRAQ5 can be excited by wavelengths from 488 to 647 nm. For microscopy/imagin…
The Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) is a cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldin A and monensin. PMA and ionomycin treatment can induce activation of many cell types to produce cytokines. Brefeldin A and Monensin result in the accumulation of secreted proteins in the endoplasmic reticulum and Golgi apparatus. The Cell Stimulation Cocktail (plus protein transp…
The Phytohemagglutinin-L (PHA-L) Solution (500X) is a ready-to-use solution of PHA-L in aqueous buffer. PHA is a mixture of lectins isolated from the red kidney bean, Phaseolus vulgaris. PHA-L is the fraction of PHA that exhibits strong leukoagglutinating and mitogenic activity, but very little erythroagglutinating activity. This reagent is intended for use in in vitro activation of human and mouse leukocytes.
The Concanavalin A (Con A) Solution (500X) is a ready-to-use solution of Con A in aqueous buffer. Con A, isolated from the Jack bean, Canavlia ensiformis, is a lectin that binds alpha-D-glucose and alpha-D-mannose moieties found in various glycoproteins, glycolipids, and sugars and is a potent leukocyte mitogen. This reagent is intended for use in in vitro activation of human and mouse leukocytes.
CellEvent Caspase-3/7 Green Ready Flow Reagent enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The product includes 2 reagents: the novel fluorogenic substrate CellEvent Caspase-3/7 Green Detection Reagent and SYTOX™ AADvanced™ Dead Cell Stain.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteExceptionally bright green fluorescen…
