Quantitate mRNA capping efficiency (percent capped RNA content) using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective, benchtop method useful for QC as an alternative to HPLC and LC-MS analysis.
Quantitate the percentage of mRNA with Cap 1 vs Cap 0 5’-cap structures using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective benchtop method for QC as an alternative to mass spec analysis.
All-in-one kit to produce pseudouridine-containing-mRNA (Ψ-mRNA). Combine the benefits of high-yield transcription with post-transcriptional capping and tailing. Produce large amounts of Ψ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells by using Ψ-mRNAs.
Specially formulated for maximum yields of N1-methyl-pseudouridine-containing, in vitro transcribed RNA (N1meΨ-RNA). Produce up to 160 µg of N1meΨ-RNA from 1 µg of template in 1 hour. Easily scale up reactions to produce milligram amounts of N1meΨ-RNA. Induce lower innate immune responses in mammalian cells by using N1meΨ-mRNAs.
