ZymoPURE™  Plasmid Miniprep Kit by Zymo Research

"Very effective and easy to use."

"The kit is very simple and easy to use to isolate plasmid DNA from the bacterial culture. The protocol is very simple and yields high purity plasmid DNA."

"The kit is very easy to use and cost effective!"

"The ZymoPure mini prep kit produces great quality DNA particularly for the time devoted to the prep. Transfects and even electroporates cells and tissues well."

"The product was extremely effective in isolating the plasmids with no/low contamination."

"This plasmid mini prep kit is not only easy to use with clear instructions, but provides pretty good results. I isolate plasmids for further characterization and usually do not have issues with obtaining mostly pure plasmids using this kit."

"We switched to the Zymo kit because it gave us low RNAse carryover in our miniprep DNA. We wanted to be able to linearize the DNA and use it directly in an IVT reaction without an additional cleanup step. Our previous kit had too much RNAse carryover which would cause the mRNA made in the IVT to degrade. We spent a lot of time cleaning up the linearized DNA and losing half before discovering this kit."

"This product is very useful and includes a detailed, straightforward protocol."

"The kit is very easy to use. We can get pure plasmids within a short time with a low cost per sample. It is a good choice as a routine kit for plasmid isolation for high requirement downstream experiments, like transfection and sequencing."

"Like other Zymo kits we've used, this one is very quick to learn and use. It gives consistent quantity and quality of plasmids isolated from bacteria. It is not the cheapest available kit but for ease of use it is worth the extra money if you can spare it. Also like other Zymo kits, after the final elution it's a good idea to let your tubes sit open at RT for 5 minutes or so to let residual ethanol evaporate. I would not recommend this kit for people unfamiliar with plasmid preps in general - the instruction manual is hard to read and could use some editing (also the illustrations of colored buffers are printed in black and white)."

"We tested out several different types of transfection grade minipreps. This kit from Zymo gives the best yield, by far, of any of the kits that we tested, and the DNA can easily be used for transfections. We get enough from a single miniprep to do several Sanger sequencing reactions, and still have enough remaining to do a small transfection without the need to re-prep."

"This kit was used repeatedly in a classroom setting, making is easy for students to learn about the different solutions in the kit. Colors were helpful to keep students oriented and prevent them from making mistakes. Great yields of relatively pure plasmids were extracted that the students were then able to work with throughout the entire semester."

"ZymoPURE Miniprep system is very easy and fast to use and is an improvement from their previous plasmid prep kit. I would recommend giving the trial version a try as a comparison."

"The multi-coloured buffers made it easy to ensure the correct one was added to each tube. The amount recovered and the quality was similar to that obtained from a side-by-side comparison with NEB Monarch Plasmid Miniprep kit and an Invitrogen PureLink Quick Plasmid Miniprep kit. However, the Zymo kit did take a few minutes longer due to 2 extra steps, and required 1 additional Eppendorf tube per miniprep. I do like that the binding membrane has a relatively small surface area yet appears to be highly effective at binding plasmid DNA, which allows for recovery in a minimal volume."

"We used to use the ZR Classic Miniprep kit and we were really happy with it. Then we switched to the ZymoPure Miniprep kit because it looked like this newer kit, being more expensive, it would produce even better quality and yield. However, every member in both labs I work for are not enjoying the longer times that the new kit takes, and we haven't had good luck with the recovery either. Also, with the ZR Classic kit, we were able to get plasmid from many other strains. besides E. coli, but the ZymoPure Kit doesn't pellet these strains well. Overall, I would not recommend this product. The ZR Classic kit is a far better alternative, being cheaper, faster, and can be used in a wider ranger of cells."

"The Miniprep kit was quite effective for the extraction of the plasmid! Easy to use and quick. The result at the end reveal a good clean sample with good yield."

"High quality miniprep kit with easy to follow written and visual instructions. Reproducible high yields, with pure DNA extraction. Options for both vacuum and centrifugation purification, both work extremely well. Color coding of steps ensures that you always go in the correct order! Great value for the money."