Status:
ReviewerMember since: 2023
Organization: Wayne State University
Can't live without this prep!
Application Area: Analyze plasmid DNA from cloning experiments with MRSA mutation candidates.
"I use the Zymo mini-prep for plasmid DNA isolation and it is the easiest prep I've worked with. All of the components needed along with the tubes and spin columns are included in the kit. It's like making premium brownies in your own kitchen. The prep always works, the DNA is clean enough to do anything I want with it and it always turns out great. The yield is good and the prep is quick... depending on how many samples I am trying to make, I spend under two hours isolating my DNA."
Status:
ReviewerMember since: 2022
Organization: Altus Enterprises, Inc
Great product, happy to have switch to Zymo from Invitrogen.
Application Area:Plasmid purification for mutagenesis and transfection
"The whole protocol from before spinning down culture to final elution is ~2.5 hours for 20 samples. Cell Source: Thermo TOP10 cells Culture Volume: 5mL Very easy to use, there is a lot of flexibility in timing of steps if something comes up while processing. The methodology is well thought out and resilient to user error. Product states that one can expect 100ug, but from user experience when the process is handled with care we have been able to purify ~200ug from 5mL overnight culture. As long as the cells have no problems with growth, 75-100ug is a reliable target that should be reached with every prep. User failure rate for our group (<20ug) is <5%."
Status:
ReviewerMember since: 2022
Organization: University of Florida
Great results. Easy to use.
Application Area:Purification of transfection-grade plasmid DNA
"Great results from this product. Provides high yields and transfection-grade plasmid DNA using spin columns. Recent protocol update no longer requires one of the reagents to be ice cold which saves time."
Status:
ReviewerMember since: 2022
Organization: Biomolecular Discovery Services
Highly recommend.
Application Area:Transfection
"High quality and yield of plasmid DNA. I use the DNA for transfections with great success. Very happy with the quality of this product, especially considering the low cost of the kit. Highly recommend!"
Status:
ReviewerMember since: 2022
Organization: Cedars-Sinai Medical Center
Considering other kits if Zymo does not put more effort into the kit!
Application Area:Plasmid DNA extraction
"I have purchased this large kit 3 times already. I have found it is easy to use with clear protocol instructions. However, in this last kit I noted many columns were missing the purple insert, and sometimes the column filter would float upon addition of buffer. I also noted they do not add 10% extra to some of their buffers and the kit comes with exact volumes for the reactions. Note this is the case with the Wash 1 buffer. I am surprised given most companies will include extra solutions to account for pipetting errors. Not sure I will keep buying the kit if these two things are not fixed. Value for the money is great!"
Status:
ReviewerMember since: 2021
Organization: SDSMT
Wonderful Kit
Application Area:Plasmid extraction from bacteria
"The kit is effective in extracting good quality plasmids E.coli, Thermophiles, and other microbes. The results are reproducible."
Status:
ReviewerMember since: 2021
Organization: IBER-BAS
Would highly recommend.
Application Area:Molecular ecology
"Easy to use, high quality purified prep at the end, excellent for further visualisation or transfection."
Status:
Advanced ReviewerMember since: 2021
Organization: St jude childrens research hospital
Easy, short time and high yield of plasmid DNA.
Application Area:High quality plasmid DNA
"The kit is very simple and easy to use to isolate plasmid DNA from the bacterial culture. The protocol is very simple and yields high purity plasmid DNA."
Status:
ReviewerMember since: 2021
Organization: University of California, Irvine
Our first pick for plasmid miniprep!
Application Area:DNA purification
"The kit is very easy to use and cost effective!"
Status:
Advanced ReviewerMember since: 2019
Organization: Unviersity of Chicago
High quality DNA at a smaller scale.
Application Area:Plasmid purification
"The ZymoPure mini prep kit produces great quality DNA particularly for the time devoted to the prep. Transfects and even electroporates cells and tissues well."
Status:
ReviewerMember since: 2021
Organization: Thunder Biotech
Very useful product!
Application Area:Isolating plasmids for sequencing to use in transformation
"The product was extremely effective in isolating the plasmids with no/low contamination."
Status:
ReviewerMember since: 2021
Organization: AcuImmune
10/10 would recommend for plasmid isolation.
Application Area:Isolation of plasmids for further characterization
"This plasmid mini prep kit is not only easy to use with clear instructions, but provides pretty good results. I isolate plasmids for further characterization and usually do not have issues with obtaining mostly pure plasmids using this kit."
Status:
ReviewerMember since: 2020
Organization: Arcturus
Low RNAse levels.
Application Area:Therapeutics
"We switched to the Zymo kit because it gave us low RNAse carryover in our miniprep DNA. We wanted to be able to linearize the DNA and use it directly in an IVT reaction without an additional cleanup step. Our previous kit had too much RNAse carryover which would cause the mRNA made in the IVT to degrade. We spent a lot of time cleaning up the linearized DNA and losing half before discovering this kit."
Status:
ReviewerMember since: 2020
Organization: Cemvita Factory
Awesome product, great instruction manual!
Application Area:Plasmid purification
"This product is very useful and includes a detailed, straightforward protocol."
Status:
ReviewerMember since: 2019
Organization: Children's Hospital of Philadelphia
Given the cost, quality and usability, this is absolutely the best choice!
Application Area:Isolate plasmids for molecular cloning
"The kit is very easy to use. We can get pure plasmids within a short time with a low cost per sample. It is a good choice as a routine kit for plasmid isolation for high requirement downstream experiments, like transfection and sequencing."
Status:
Advanced ReviewerMember since: 2020
Organization: University of Idaho
Easy to use, gives consistent results, manual needs updating.
Application Area:Plasmid biology
"Like other Zymo kits we've used, this one is very quick to learn and use. It gives consistent quantity and quality of plasmids isolated from bacteria. It is not the cheapest available kit but for ease of use it is worth the extra money if you can spare it. Also like other Zymo kits, after the final elution it's a good idea to let your tubes sit open at RT for 5 minutes or so to let residual ethanol evaporate. I would not recommend this kit for people unfamiliar with plasmid preps in general - the instruction manual is hard to read and could use some editing (also the illustrations of colored buffers are printed in black and white)."
Status:
ReviewerMember since: 2016
Organization: GigaGen
Best transfection-grade miniprep kit I have used.
Application Area:Preparation of transfection grade minipreps
"We tested out several different types of transfection grade minipreps. This kit from Zymo gives the best yield, by far, of any of the kits that we tested, and the DNA can easily be used for transfections. We get enough from a single miniprep to do several Sanger sequencing reactions, and still have enough remaining to do a small transfection without the need to re-prep."
Status:
ReviewerMember since: 2019
Organization: SUNY Plattsburgh
Awesome.
Application Area:Plasmid extraction for downstream cloning applications
"This kit was used repeatedly in a classroom setting, making is easy for students to learn about the different solutions in the kit. Colors were helpful to keep students oriented and prevent them from making mistakes. Great yields of relatively pure plasmids were extracted that the students were then able to work with throughout the entire semester."
Status:
ReviewerMember since: 2019
Organization: Montana State University
Easy and fast kit.
Application Area:Purifying plasmids for transfection
"ZymoPURE Miniprep system is very easy and fast to use and is an improvement from their previous plasmid prep kit. I would recommend giving the trial version a try as a comparison."
Status:
ReviewerMember since: 2019
Organization: University of Manitoba
Resulting plasmid DNA was of good purity and ready for downstream applications.
Application Area:Molecular biology and neuroscience
"The multi-coloured buffers made it easy to ensure the correct one was added to each tube. The amount recovered and the quality was similar to that obtained from a side-by-side comparison with NEB Monarch Plasmid Miniprep kit and an Invitrogen PureLink Quick Plasmid Miniprep kit. However, the Zymo kit did take a few minutes longer due to 2 extra steps, and required 1 additional Eppendorf tube per miniprep. I do like that the binding membrane has a relatively small surface area yet appears to be highly effective at binding plasmid DNA, which allows for recovery in a minimal volume."
Status:
ReviewerMember since: 2019
Organization: North Carolina State University
Overall, I would not recommend. ZR Classic Kit is a far better alternative for us.
Application Area:Miniprep plasmids before/after cloning
"We used to use the ZR Classic Miniprep kit and we were really happy with it. Then we switched to the ZymoPure Miniprep kit because it looked like this newer kit, being more expensive, it would produce even better quality and yield. However, every member in both labs I work for are not enjoying the longer times that the new kit takes, and we haven't had good luck with the recovery either. Also, with the ZR Classic kit, we were able to get plasmid from many other strains. besides E. coli, but the ZymoPure Kit doesn't pellet these strains well. Overall, I would not recommend this product. The ZR Classic kit is a far better alternative, being cheaper, faster, and can be used in a wider ranger of cells."
Status:
ReviewerMember since: 2019
Organization: Trinity College Dublin
Great Results! Good yield at the end.
Application Area:Microbiology - strains transformation
"The Miniprep kit was quite effective for the extraction of the plasmid! Easy to use and quick. The result at the end reveal a good clean sample with good yield."
Status:
ReviewerMember since: 2019
Organization: Thomas Jefferson University
Awesome kit, easy to use and high yields.
Application Area:Isolation of plasmids for use in cloning and transfections
"High quality miniprep kit with easy to follow written and visual instructions. Reproducible high yields, with pure DNA extraction. Options for both vacuum and centrifugation purification, both work extremely well. Color coding of steps ensures that you always go in the correct order! Great value for the money."
Manufacturer Zymo Research | Available Worldwide
4.6 / 5.0 | 27 reviews