ZymoPURE™  Plasmid Miniprep Kit by Zymo Research

Manufacturer Zymo Research  |   Model: D4208T  |  Available Worldwide
4.6
/
5.0
  |  21 reviews
This plasmid miniprep kit is the fastest and simplest method to isolate endotoxin-free plasmid DNA. Get a free sample today with free shipping.


ZymoPURE™  Plasmid Miniprep Kit by Zymo Research product image
ZymoPURE Plasmid Miniprep Kits
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Average Rating: 4.6
21 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2020

  • Organization: University of Washington



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Everyday user
Rating: 4.3

  • Application Area: Plasmid clean up

"Works great!"

Review date: 16 Nov 2021 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: SDSMT



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Wonderful Kit
Rating: 4.3

  • Application Area:Plasmid extraction from bacteria

"The kit is effective in extracting good quality plasmids E.coli, Thermophiles, and other microbes. The results are reproducible."

Review date: 16 Nov 2021 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Bayer



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results.
Rating: 5.0

  • Application Area:Molecular biology

"Very good plasmid yield and price."

Review date: 16 Jun 2021 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: IBER-BAS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Would highly recommend.
Rating: 4.7

  • Application Area:Molecular ecology

"Easy to use, high quality purified prep at the end, excellent for further visualisation or transfection."

Review date: 16 Jun 2021 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: CCDM



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Very happy with this product.
Rating: 4.7

  • Application Area:Plasmid extractions

"Very effective and easy to use."

Review date: 07 Jun 2021 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Advanced Reviewer

  • Member since: 2021

  • Organization: St jude childrens research hospital



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Easy, short time and high yield of plasmid DNA.
Rating: 4.7

  • Application Area:High quality plasmid DNA

"The kit is very simple and easy to use to isolate plasmid DNA from the bacterial culture. The protocol is very simple and yields high purity plasmid DNA."

Review date: 30 May 2021 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of California, Irvine



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Our first pick for plasmid miniprep!
Rating: 4.0

  • Application Area:DNA purification

"The kit is very easy to use and cost effective!"

Review date: 26 May 2021 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Advanced Reviewer

  • Member since: 2019

  • Organization: Unviersity of Chicago



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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High quality DNA at a smaller scale.
Rating: 5.0

  • Application Area:Plasmid purification

"The ZymoPure mini prep kit produces great quality DNA particularly for the time devoted to the prep. Transfects and even electroporates cells and tissues well."

Review date: 26 May 2021 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Thunder Biotech



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Very useful product!
Rating: 5.0

  • Application Area:Isolating plasmids for sequencing to use in transformation

"The product was extremely effective in isolating the plasmids with no/low contamination."

Review date: 17 Mar 2021 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: AcuImmune



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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10/10 would recommend for plasmid isolation.
Rating: 5.0

  • Application Area:Isolation of plasmids for further characterization

"This plasmid mini prep kit is not only easy to use with clear instructions, but provides pretty good results. I isolate plasmids for further characterization and usually do not have issues with obtaining mostly pure plasmids using this kit."

Review date: 16 Mar 2021 | ZymoPURE™  Plasmid Miniprep Kit


  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Arcturus



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Low RNAse levels.
Rating: 4.7

  • Application Area:Therapeutics

"We switched to the Zymo kit because it gave us low RNAse carryover in our miniprep DNA. We wanted to be able to linearize the DNA and use it directly in an IVT reaction without an additional cleanup step. Our previous kit had too much RNAse carryover which would cause the mRNA made in the IVT to degrade. We spent a lot of time cleaning up the linearized DNA and losing half before discovering this kit."

Review date: 04 Dec 2020 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Cemvita Factory



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Awesome product, great instruction manual!
Rating: 5.0

  • Application Area:Plasmid purification

"This product is very useful and includes a detailed, straightforward protocol."

Review date: 04 Dec 2020 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Children's Hospital of Philadelphia



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Given the cost, quality and usability, this is absolutely the best choice!
Rating: 5.0

  • Application Area:Isolate plasmids for molecular cloning

"The kit is very easy to use. We can get pure plasmids within a short time with a low cost per sample. It is a good choice as a routine kit for plasmid isolation for high requirement downstream experiments, like transfection and sequencing."

Review date: 17 Jul 2020 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Advanced Reviewer

  • Member since: 2020

  • Organization: University of Idaho



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Easy to use, gives consistent results, manual needs updating.
Rating: 4.7

  • Application Area:Plasmid biology

"Like other Zymo kits we've used, this one is very quick to learn and use. It gives consistent quantity and quality of plasmids isolated from bacteria. It is not the cheapest available kit but for ease of use it is worth the extra money if you can spare it. Also like other Zymo kits, after the final elution it's a good idea to let your tubes sit open at RT for 5 minutes or so to let residual ethanol evaporate. I would not recommend this kit for people unfamiliar with plasmid preps in general - the instruction manual is hard to read and could use some editing (also the illustrations of colored buffers are printed in black and white)."

Review date: 05 Jun 2020 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: GigaGen



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Best transfection-grade miniprep kit I have used.
Rating: 5.0

  • Application Area:Preparation of transfection grade minipreps

"We tested out several different types of transfection grade minipreps. This kit from Zymo gives the best yield, by far, of any of the kits that we tested, and the DNA can easily be used for transfections. We get enough from a single miniprep to do several Sanger sequencing reactions, and still have enough remaining to do a small transfection without the need to re-prep."

Review date: 05 Jun 2020 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: SUNY Plattsburgh



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Awesome.
Rating: 5.0

  • Application Area:Plasmid extraction for downstream cloning applications

"This kit was used repeatedly in a classroom setting, making is easy for students to learn about the different solutions in the kit. Colors were helpful to keep students oriented and prevent them from making mistakes. Great yields of relatively pure plasmids were extracted that the students were then able to work with throughout the entire semester."

Review date: 13 Dec 2019 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Montana State University



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Easy and fast kit.
Rating: 4.3

  • Application Area:Purifying plasmids for transfection

"ZymoPURE Miniprep system is very easy and fast to use and is an improvement from their previous plasmid prep kit. I would recommend giving the trial version a try as a comparison."

Review date: 12 Dec 2019 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Manitoba



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
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Resulting plasmid DNA was of good purity and ready for downstream applications.
Rating: 3.3

  • Application Area:Molecular biology and neuroscience

"The multi-coloured buffers made it easy to ensure the correct one was added to each tube. The amount recovered and the quality was similar to that obtained from a side-by-side comparison with NEB Monarch Plasmid Miniprep kit and an Invitrogen PureLink Quick Plasmid Miniprep kit. However, the Zymo kit did take a few minutes longer due to 2 extra steps, and required 1 additional Eppendorf tube per miniprep. I do like that the binding membrane has a relatively small surface area yet appears to be highly effective at binding plasmid DNA, which allows for recovery in a minimal volume."

Review date: 05 Jun 2019 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: North Carolina State University



  • Ease of use
    2 out of 5
    After sales service
    5 out of 5
    Value for money
    2 out of 5
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Overall, I would not recommend. ZR Classic Kit is a far better alternative for us.
Rating: 3.0

  • Application Area:Miniprep plasmids before/after cloning

"We used to use the ZR Classic Miniprep kit and we were really happy with it. Then we switched to the ZymoPure Miniprep kit because it looked like this newer kit, being more expensive, it would produce even better quality and yield. However, every member in both labs I work for are not enjoying the longer times that the new kit takes, and we haven't had good luck with the recovery either. Also, with the ZR Classic kit, we were able to get plasmid from many other strains. besides E. coli, but the ZymoPure Kit doesn't pellet these strains well. Overall, I would not recommend this product. The ZR Classic kit is a far better alternative, being cheaper, faster, and can be used in a wider ranger of cells."

Review date: 05 Jun 2019 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Trinity College Dublin



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great Results! Good yield at the end.
Rating: 5.0

  • Application Area:Microbiology - strains transformation

"The Miniprep kit was quite effective for the extraction of the plasmid! Easy to use and quick. The result at the end reveal a good clean sample with good yield."

Review date: 05 Jun 2019 | ZymoPURE™  Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Thomas Jefferson University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Awesome kit, easy to use and high yields.
Rating: 5.0

  • Application Area:Isolation of plasmids for use in cloning and transfections

"High quality miniprep kit with easy to follow written and visual instructions. Reproducible high yields, with pure DNA extraction. Options for both vacuum and centrifugation purification, both work extremely well. Color coding of steps ensures that you always go in the correct order! Great value for the money."

Review date: 05 Jun 2019 | ZymoPURE™  Plasmid Miniprep Kit
The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume.