ZymoBIOMICS™ Microbial Community DNA Standard by Zymo Research
Status:
Reviewer
Member since: 2019
Organization: Erasmus MC
-
- Ease of use
- After sales service
- Value for money
Very recommendable to assess microbiome workflows from 'bench-to-bedside'.
Application Area: Quality control during microbiome analysis
"I use the ZymoBIOMICS Microbial DNA Standard as a separate sample during my 16S rRNA gene library preparation and sequencing experiments. The DNA standard contains a known amount of eight bacterial and two fungal species genomes to assess the performance of the microbiome pipelines from the very beginning until the data analysis step. It is very straightforward to use, and I have obtained accurate and reproducible results so far. I have also used this for optimizing PCR experiments, obtaining good results as well. Just a 'heads-up' for new users: when performing taxonomic assignation of the amplicon sequence variants obtained from the DNA Standard sample, double-check your results with more than a single 16S rRNA gene database in case you are missing any of the microbial species contained in the DNA Standard. I was using the Human Oral Microbiome Database (HOMD) for assigning taxonomy and I was missing Salmonella enterica on the DNA Standard sample. I double-checked the non-assigned (NA) amplicon sequence variants using standard nucleotide blast against the nucleotide collection database from the National Center for Biotechnology Information (NCBI) and, indeed, they were assigned to S. enterica."
Review date: 11 Dec 2019 | ZymoBIOMICS™ Microbial Community DNA Standard
Status:
Reviewer Member since: 2019
Organization: UC Berkeley
-
- Ease of use
- After sales service
- Value for money
Great mock for inferring community composition with NGS.
Application Area:Next Generation Sequencing
"The community mock is always retrieved in the expected relative abundances. It provides a lot of certainty for the composition of my samples and the quality of sequencing. Examining the accuracy of the mock provides information on issues such as over- or under-representation of certain sequences and guides the subsequent filtering steps I need to take in order to accurately analyze my own samples."
Review date: 06 Jun 2019 | ZymoBIOMICS™ Microbial Community DNA Standard
Status:
Reviewer Member since: 2019
Organization: Caltech/ JPL
-
- Ease of use
- After sales service
- Value for money
Very useful product. We use it in each metagenome library.
Application Area:Standard for metagenome library construction
"The ZymoBIOMICS Microbial Community Standard is used by my lab to assess DNA extraction bias and sequencing errors. It has almost become a 'gold standard' in our lab. I have recommended it to several colleagues and collaborators."
Review date: 05 Jun 2019 | ZymoBIOMICS™ Microbial Community DNA Standard
Status:
Reviewer Member since: 2019
Organization: University of Illinois
-
- Ease of use
- After sales service
- Value for money
A pleasant experience so far. I look forward to working with them in the future.
Application Area:Microbial analysis
"So far the service has been timely, knowledgable and affordable. I haven't actually received my results, but I have had a pleasant experience so far."
Review date: 05 Jun 2019 | ZymoBIOMICS™ Microbial Community DNA Standard
ZymoBIOMICS™ Microbial Community DNA Standard is a mixture of genomic DNA extracted from pure cultures of eight bacterial and two fungal strains. Genomic DNA from each pure culture was extracted and quantified before mixing. The GC content of the containing genomes covers a range from 15% to 85%. The microbial standard is accurately characterized and contains negligible impurities ( This standard is also ideal to help users construct and optimize workflows, e.g. controlling PCR chimera rate and noise in the library preparation of 16S rRNA gene targeted sequencing, and assessing GC bias in sequencing coverage of shotgun metagenomic sequencing.
Product Overview
