Member since: 2019
Great, high concentration of DNA!
Application Area: Purify PCR products for cloning
"I used the Wizard SV Gel and PCR Clean-Up kit to purify PCR products I needed to make a MoClo basic part. The kit was easy to use, quick and gave me a high concentration of DNA. It was also very easy to purchase, we have a fridge containing the kit at UCL Bioscience stores."
Member since: 2010
Organization: Braskem, S.A.
"The Wizard PCR and gel purification kit is our go-to solution for fast a reliable purification of DNA for cloning applications. I have successfully used the purified DNA for In-fusion, Gibson and Golden Gate cloning applications. "
Member since: 2009
Organization: Fundación Caubet-Cimera Centro Internacional de Medicina
Application Area:Purify genomic DNA from mammalian cell lines
"The product is versatile, efficient for both gel and column applications. The protocol is very straightforward to follow and results are significantly better than analogous products from other manufacturers."
The Wizard® SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100bp to 10kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests.
Up to 95% recovery is achieved depending upon the DNA fragment size. PCR products are commonly purified to remove excess nucleotides and primers. This membrane-based system, which can bind up to 40μg of DNA, allows recovery of isolated DNA fragments or PCR products in as little as 15 minutes, depending on the number of samples processed.
Wizard® SV Gel and PCR Clean-Up System Features & Benefits: