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Ultra-Deep Microbiome Prep

Microbial DNA enrichment and isolation from fluids and tissue samples

Molzym GmbH & Co. KG

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Description

The DNA-free Ultra-Deep Microbiome Prep kits combine the depletion of host DNA and extraction of enriched microbial DNA from fluid samples and tissues. This allows significantly deeper sequencing compared to conventional DNA isolation and increases the sensitivity of many analytical methods as PCR, arrays and diverse NGS approaches e.g. microbiome and metagenomic analyses. The kits provide DNA-free reagents and consumables for the depletion of human/animal DNA and the isolation of enriched microbial DNA.

Benefits:

  • Depletion of non-target, human or animal DNA (MolYsis™ technology)
  • Selective enrichment of bacteria and fungi from body fluids and tissues
  • Validated for a broad-range lysis of bacteria and fungi
  • DNA-free reagents and consumables
  • Sample volumes from 0.2-10 ml

Kits are available for small (0.2-1 ml) and large (1-10 ml) sample volumes. The Ultra-Deep Microbiome Prep kits can be used for a variety of sample types e.g. whole blood, body fluids, sputum, urine, human and animal tissues and biofilms. Microorganisms need to be intact for a successful isolation. Please check out Molzym’s DNA-free PCR reagents and assay solutions for the contamination-free and sensitive sample analysis.

Brochures

Unleash the power of mNGS to identify infectious agents in complex human samples

A major challenge to enhance the use of mNGS for the identification of infectious agents is linked to the vast excess of human DNA, which negatively impacts sequencing results, sensitivity and the overall workflow. In this webinar, we will discuss how human DNA depletion by MolYsis™ can help overcome such limitations, improve sequencing depth and quality, and reduce the time and costs of analysis.

Key learning objectives:

  • Learn more about the challenges and opportunities of mNGS in clinical infection diagnosis
  • Learn how to increase sequencing depth and quality of microbial reads by depleting human DNA from body fluids, tissues and swabs
  • Learn how MolYsis™ impacts the number of sequencing reads, sequencing time and overall data cost
  • Learn how to facilitate the mNGS workflow for the identification of bacteria and fungi

Who should attend?

  • Lab directors, lab managers and quality managers
  • Medical laboratory scientists and technicians
  • Molecular microbiologists
  • Clinical lab scientists
  • Clinicians, infectious disease specialists and pathologists
  • Researchers, microbiologists and biotechnologists

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

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