Products & ReviewLife Sciences

MolYsis™ Complete5

Host DNA depletion and microbial DNA isolation

Molzym GmbH & Co. KG

The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our  Product Directory.

Ease of Use
After Sales Service
Value for Money
Write your own review

Host DNA often negatively influences the sensitivity and specificity of molecular analysis of microorganisms in human and animal samples. The proprietary MolYsis™ technology provides a solution for the depletion of non-target host DNA and enrichment and isolation of bacterial and fungal DNA from intact microorganisms. All reagents and consumables provided with the kits are DNA-free ensuring pure DNA without reagent-borne contaminations. During the MolYsis™ process, human or animal cells are selectively lysed and the free DNA is degraded by a DNase treatment. Afterwards, a reagent called BugLysis is used for a broad-range lysis of Gram-positive and Gram-negative bacteria and fungi. The microbial DNA is purified with a column-based bind-wash-elute procedure providing pure, enriched microbial DNA. Applications comprise next generation sequencing, amplicon cloning, real-time PCR, Sanger sequencing and DNA array analysis of microorganisms.

Benefits:

  • Depletion of non-target, human or animal DNA
  • Selective enrichment of bacteria and fungi
  • Validated for a broad-range lysis of bacteria and fungi
  • DNA-free reagents and consumables
  • Suitable for fluids of 0.2-5 ml sample volumes

MolYsis™ Complete5 is validated for a variety of human and animal body fluids.

Product Overview

Links