THUNDER Imager

Deconvolution is a computational method used to restore the image of the object that is corrupted by the point spread function (PSF) along with sources of noise. In this technical brief, learn how the deconvolution algorithm offered by Leica Microsystems allows you to overcome losses in image resolution and contrast in widefield (WF) fluorescence microscopy due to the wave nature of light and the diffraction of light by optical elements. Explore the methods of user-controlled or automated deconvolution to see and resolve more structural detail.

Live-cell imaging enables the visualization of living cells in real time. Using live-cell microscopy techniques, researchers can gain valuable insights into the biological relevance of physiological processes such as cell localization, migration, growth, and dynamic cellular interactions.

This eBook provides a comprehensive guide to the different live-cell imaging techniques available, highlights key considerations to help maximize your experimental success, and presents solutions that can address even the toughest imaging applications.

Download your guide free today for:

• An introduction to live-cell imaging
• Important considerations, techniques, and applications
• Expert insight into the preservation of cell homeostasis
• The principles of widefield imaging and the problem of out-of-focus light

Commonly in widefield (WF) microscopy, the imaging of objects in the object plane results in captured images that are compounded by image haze (referred to as background noise). The presence of background noise in an image obscures structural features from being resolved. Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise from WF images are rolling ball and sliding paraboloid. Recently, Leica Microsystems introduced their own background subtraction method called Instant Computational Clearing (ICC), which is present in all Leica THUNDER WF imaging platforms. Find out more about how the technology works.

THUNDER Imagers provide you with a solution for advanced 3D cell culture assays, whether you want to study stem cells, spheroids, or organoids. Watch this video to discover the key advantages that the THUNDER Imager 3D Live Cell & 3D Cell Culture offer.

Discover the new THUNDER Imager from Leica Microsystems that offers to deliver a new benchmark for core life science applications. The system's Computational Clearing method enables you to capture images that are clearer than ever before with widefield observation, differentiating between signal and background, giving images so clear you can separate single cells from each other and use the images for downstream analysis.

The partnership will incorporate CrestOptics' CICERO spinning disk imaging unit within Leica Microsystems' THUNDER Imager Cell system

Hear from leading experts and explore tools to advance your work using 3D cell culture

Dr. Falco Krüger discusses the challenges of 3D cell culture imaging, enabling technologies and the future of AI microscopy

Experience the solutions new functions with a free trial on the Aivia web platform

The new solution promises to accelerate experimental success and improve process reliability as industry looks to faster drug discovery post-Covid-19

From 3D Culture Systems to Spheroids – break into three-dimensional (3D) cell culture with the latest methods and resources

Find all the latest developments in antibody engineering and June’s top content from our drug discovery community

In this article, discover how Computational Clearing technology from Leica Microsystems quickly resolves the longstanding challenge of blurry contrast when imaging thick sections

The THUNDER imager family enables users to decode 3D biology in real-time*